Corn stovers are rich in carbohydrates and can be used by anaerobic bacteria to produce hydrogen by fermentation. In the present study, using hydrogen production as the main experimental index, the effect of different influential factors on hydrogen production from corn stover saccharification and fermentation was studied, using the response surface method BBD model. The significance of interactions between different influential factors on hydrogen production by simultaneous saccharification and fermentation of corn stover material were investigated and optimized. Results showed that there were several factors affecting simultaneous saccharification fermentative hydrogen production from corn stover, including substrate concentration, inoculation amount, pH value and enzyme concentration. In linear terms, substrate concentration had the greatest influence on hydrogen production by anaerobic simultaneous saccharification and fermentation. In terms of multi-factor interactions, the interaction between pH and enzyme concentration was the most significant. The optimal hydrogen production conditions established from the BBD model were as follows: substrate concentration of 25 mg/mL, inoculation amount proportion of 32.62%, initial pH value of 6.50 and enzyme concentration of 172.08 mg/g, resulting in the maximum hydrogen production of 55.29 mL/g TS. The actual maximum hydrogen production reached 56.66 mL/g TS, with these experimental results consistent with the predicted value established from equation fitting. This study provides a reference for hydrogen production by anaerobic synchronous saccharification fermentation using corn stover as substrate and lays a foundation and provides technical support for the industrialization of biological hydrogen production using corn stover as substrate.

中文翻译：

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玉米秸秆同步糖化发酵产氢的统计优化

玉米秸秆富含碳水化合物，可被厌氧菌利用发酵产生氢气。本研究以产氢量为主要实验指标，采用响应面法BBD模型，研究了不同影响因素对玉米秸秆糖化发酵产氢量的影响。研究并优化了不同影响因素对玉米秸秆原料同步糖化发酵产氢的交互作用的意义。结果表明，影响玉米秸秆同步糖化发酵产氢的因素有底物浓度、接种量、pH值和酶浓度等。从线性角度来看，底物浓度对厌氧同时糖化发酵产氢的影响最大。就多因素相互作用而言，pH和酶浓度之间的相互作用最为显着。BBD模型建立的最佳产氢条件为：底物浓度25 mg/mL、接种量比例32.62%、初始pH值6.50、酶浓度172.08 mg/g，产氢量最大为55.29 毫升/克 TS。实际最大产氢量达到56.66 mL/g TS，实验结果与方程拟合建立的预测值一致。本研究为玉米秸秆为基质的厌氧同步糖化发酵制氢提供了参考，为玉米秸秆为基质的生物制氢产业化奠定了基础并提供了技术支撑。