In this study, a total of six fungal samples were isolated from apple, strawberry and orange pulp. DNA sequence analysis was used as molecular identification method. ITS region was aligned in DNA sequence analysis, and an algorithm sequence similarity was done using BLAST (Basic Local Alignment Search Tool) program to identify these isolates. All the six isolates were identified as Aspergillus fumigates. The total lipid content was varied in the isolates which were ranged from 29.4 to 21.0 mg/100 ml. Moreover, the obtained lipid form mycelium biomass of the isolates was transesterified by a base catalyst. The methyl esters were analyzed by using GC-MS. GC-MS Spectrometry revealed the presence of different fatty acids with long chain (C11:0, C15:0, C17:1, C18:2, C16:1). High efficiency biodiesel can be obtained using long-chain fatty acids. Fatty acid profiles of A. fumigatus isolated from different fruit pulps have confirmed its potentiality as well as showed the beneficial utilization of these fatty acids for biodiesel production.

中文翻译：

---

使用 GC-MS 光谱法对从用于生物柴油生产的果肉中分离的烟曲霉进行脂肪酸甲酯分析。

在这项研究中，从苹果、草莓和橙子果肉中总共分离出了六种真菌样品。DNA序列分析用作分子鉴定方法。ITS 区域在 DNA 序列分析中进行比对，并使用 BLAST（基本局部比对搜索工具）程序进行算法序列相似性来识别这些分离株。所有六个分离株均被鉴定为熏曲霉。分离株中的总脂质含量变化范围为 29.4 至 21.0 mg/100 ml。此外，所获得的分离物的脂质形式菌丝体生物质通过碱催化剂进行酯交换。使用GC-MS分析甲酯。GC-MS 光谱分析显示存在不同的长链脂肪酸（C11:0、C15:0、C17:1、C18:2、C16:1）。利用长链脂肪酸可以获得高效生物柴油。从不同果肉中分离的烟曲霉的脂肪酸谱证实了其潜力，并显示了这些脂肪酸在生物柴油生产中的有益利用。