Clinical interest into the function of tuft cells in human intestine has increased in recent years. However, no quantitative study has examined intestinal tuft cells in pathological specimens from patients. This study quantified tuft cell density by using a recently identified marker, specific for tyrosine phosphorylation (pY1798) of girdin (also known as CCDC88A or GIV) in the duodenum of pediatric patients. Deidentified sections with pathological diagnosis of acute duodenitis, ulcer, or celiac disease, and age-matched normal control were analyzed under double-blind conditions. Immunostaining for pY1798-girdin demonstrated the distinct shape of tuft cells with and filopodia-like basolateral membrane structure and a small apical area, which densely expressed gamma-actin. As compared to normal tissues, the specimens diagnosed as celiac disease and duodenal ulcer had significantly fewer tuft cell numbers. In contrast, acute duodenitis showed varied population of tuft cells. The mucosa with severe inflammation showed lower tuft cell numbers than the specimens with none to mild inflammation. These results suggest that loss of tuft cells may be involved in prolonged inflammation in the duodenal mucosa and disrupted mucosal integrity. pY1798-girdin and gamma-actin are useful markers for investigating the distribution and morphologies of human intestinal tuft cells under healthy and pathological conditions.

中文翻译：

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患有急性和慢性肠病的儿科患者十二指肠簇状细胞的分布发生了改变。

近年来，临床对人体肠道簇状细胞功能的兴趣有所增加。然而，没有定量研究检查过患者病理标本中的肠簇细胞。这项研究通过使用最近发现的一种标记物来量化簇绒细胞密度，该标记物对儿科患者十二指肠中 girdin（也称为 CCDC88A 或 GIV）的酪氨酸磷酸化 (pY1798) 具有特异性。在双盲条件下分析经病理诊断为急性十二指肠炎、溃疡或乳糜泻的已鉴定切片以及年龄匹配的正常对照。pY1798-girdin 的免疫染色表明簇状细胞具有独特的形状，具有丝状伪足样基底外侧膜结构和小的顶端区域，其密集表达 γ-肌动蛋白。与正常组织相比，被诊断为乳糜泻和十二指肠溃疡的标本的簇状细胞数量明显减少。相反，急性十二指肠炎显示不同的簇状细胞群。具有严重炎症的粘膜显示出比没有或轻度炎症的标本更低的簇状细胞数量。这些结果表明，簇状细胞的丢失可能与十二指肠粘膜的长期炎症和粘膜完整性破坏有关。pY1798-girdin 和 gamma-actin 是研究健康和病理条件下人类肠道簇细胞分布和形态的有用标记。这些结果表明，簇状细胞的丢失可能与十二指肠粘膜的长期炎症和粘膜完整性破坏有关。pY1798-girdin 和 gamma-actin 是研究健康和病理条件下人类肠道簇细胞分布和形态的有用标记。这些结果表明，簇状细胞的丢失可能与十二指肠粘膜的长期炎症和粘膜完整性破坏有关。pY1798-girdin 和 gamma-actin 是研究健康和病理条件下人类肠道簇细胞分布和形态的有用标记。