A sensitive sensing method was developed for the determination of alkaline phosphatase (ALP) activity based on gold nanocluster (Au NC)-catalyzed luminol–H₂O₂ chemiluminescent (CL) reaction. The CL signal of luminol–H₂O₂–Au NCs can be quenched by ascorbic acid, which was the product of magnesium ascorbyl phosphate (MAP) hydrolysis reaction catalyzed by ALP. The proposed sensing platform showed convenient, sensitive and selective detection of ALP in the range of 0.0027 – 1.3890 U L⁻¹, with the detection limit of 0.0026 U L⁻¹. The broad detection linear range and ultra-high sensitivity were inherited from the efficient free radical scavenging capability of ascorbic acid on the luminol–H₂O₂–Au NCs CL reaction. The CL sensing platform was applied to the detection of ALP activity in serum samples. We believe that this sensing platform is a universal CL strategy for ALP detection because ascorbic acid is an efficient CL quencher for many CL reactions.

_{开发了一种基于金纳米簇（Au NC）催化的鲁米诺-H 2} O ₂化学发光（CL）反应的灵敏传感方法，用于测定碱性磷酸酶（ALP）活性。鲁米诺-H ₂ O ₂ -Au NCs的CL信号可以被抗坏血酸猝灭，抗坏血酸是ALP催化的抗坏血酸磷酸镁(MAP)水解反应的产物。所提出的传感平台对 0.0027 – 1.3890 UL ^-1范围内的 ALP 进行了方便、灵敏和选择性的检测，检测限为 0.0026 UL ^-1。宽检测线性范围和超高灵敏度继承了抗坏血酸对鲁米诺-H ₂ O ₂ -Au NCs CL 反应的高效自由基清除能力。将CL传感平台应用于血清样品中ALP活性的检测。我们相信该传感平台是用于 ALP 检测的通用 CL 策略，因为抗坏血酸是许多 CL 反应的有效 CL 猝灭剂。