Abstract

High-fat diet (HFD)-feeding induces changes in the microbiome and increases intestinal permeability by impairing tight junction (TJ) protein function, which may explain the insulin resistance (IR) and associated pathologies. We aimed to determine the effects of resveratrol (RES) on the gut microbiome and intestinal TJ proteins. Results showed that RES administration improved the lipid profile, and ameliorated the endotoxemia, inflammation, intestinal barrier defect and glucose intolerance in the HFD-fed mice. Furthermore, it modified the gut microbial composition, reducing the proportion of Firmicutes and the Firmicutes-to-Bacteroidetes ratio. Moreover, Verrucomicrobia and Akkermansia were much more abundant in the HFD + RES group. RES also significantly reduced the abundance of Bilophila and Ruminococcus. These findings suggest that RES may be useful for the treatment of IR and associated metabolic diseases.

摘要

高脂饮食（HFD）喂养会通过破坏紧密连接（TJ）蛋白质功能来诱导微生物组变化并增加肠通透性，这可能解释了胰岛素抵抗（IR）和相关病理。我们旨在确定白藜芦醇（RES）对肠道微生物组和肠道TJ蛋白的影响。结果表明，RES给药改善了喂食HFD的小鼠的脂质分布，并改善了内毒素血症，炎症，肠屏障功能障碍和葡萄糖耐量下降。此外，改性的肠道微生物组合物，硬壁菌减少和厚壁菌门到比例-拟杆菌比率。此外，HFD + RES组的疣状微生物和阿克曼病更为丰富。RES也显着降低了Bilophila和瘤胃球菌属。这些发现表明，RES可用于治疗IR和相关的代谢性疾病。