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Lactococcus protaetiae sp. nov. and Xylanimonas protaetiae sp. nov., isolated from gut of larvae of Protaetia brevitarsis seulensis.
Antonie van Leeuwenhoek ( IF 1.8 ) Pub Date : 2020-04-18 , DOI: 10.1007/s10482-020-01413-6
Jun Heo 1 , Soo-Jin Kim 1 , Mi Ae Kim 2 , Tomohiko Tamura 3 , Satomi Saitou 3 , Moriyuki Hamada 3 , Jeong-Seon Kim 1 , Seung-Beom Hong 1 , Soon-Wo Kwon 1
Affiliation  

Two bacterial strains, designated 2DFWM-2T and FW10M-9T, were isolated from gut of larva of Protaetia brevitarsis seulensis grown at the National Institute of Agricultural Sciences, Wanju-gun, South Korea. 16S rRNA and rpoB gene sequences showed that strain 2DFWM-2T formed a separate branch with Lactococcus allomyrinae 1JSPR-7T in the genus Lactococcus, adjacent to a group of Lactococcus lactis subspecies. ANI and dDDH values between 2DFWM-2T and Lactococcus allomyrinae 1JSPR-7T were 93.30% and 53.20%, respectively. Based on the 16S rRNA gene sequence analysis, strain FW10M-9T was classified into the genus Xylanimonas revealing 96.9-98.5% sequence similarities with the Xylanimonas species. The ANI values of strain FW10M-9T with the closely species Xylanimonas pachnodae NBRC 107786T, Xylanimonas allomyrinae 2JSPR-7T, Isoptericola variabilis JCM 11754T and Xylanimonas cellulosilytica DSM 15894T was 81.5%, 81.2%, 81.0% and 84.1%, respectively, and the dDDH values estimated by GGDC was 24.3%, 24.3%, 29.3% and 28.1%, respectively. On the basis of the phenotypic and genotypic data, it is proposed that strain 2DFWM-2T represents a novel species of the genus Lactococcus, for which the name Lactococcus protaetiae sp. nov. is proposed, and the type strain is 2DFWM-2T (= KACC 19320T = NBRC 113069T). And, strain FW10M-9T represents a novel species of the genus Xylanimonas, for which the name Xylanimonas protaetiae sp. nov. is proposed, and the type strain is FW10M-9T (= KACC 19331T = NBRC 113053T).

中文翻译:

丙酸乳球菌 十一月 和Xylanimonas protaetiae sp。十一月,从Protaetia brevitarsis seulensis幼虫的肠道分离。

从韩国万州郡国立农业科学研究所生长的短节霉菌幼虫的肠道中分离出两种细菌菌株,命名为2DFWM-2T和FW10M-9T。16S rRNA和rpoB基因序列表明,菌株2DFWM-2T与乳酸乳球菌1JSPR-7T在乳酸乳球菌属中形成了一个独立的分支,与一组乳酸乳球菌亚种相邻。2DFWM-2T和异链球菌乳杆菌1JSPR-7T之间的ANI和dDDH值分别为93.30%和53.20%。根据16S rRNA基因序列分析,菌株FW10M-9T被分类为Xylanimonas属,显示与Xylanimonas物种96.9-98.5%的序列相似性。FW10M-9T菌株的近距离繁殖力(Xylanimonas pachnodae NBRC 107786T,Xylanimonas allomyrinae 2JSPR-7T)的ANI值 异鳞鳞茎JCM 11754T和Xylanimonas cellulosilytica DSM 15894T分别为81.5%,81.2%,81.0%和84.1%,GGDC估计的dDDH值分别为24.3%,24.3%,29.3%和28.1%。根据表型和基因型数据,建议菌株2DFWM-2T代表乳球菌属的一个新物种,其名称为Protaetiae sp.。十一月 建议的类型应变为2DFWM-2T(= KACC 19320T = NBRC 113069T)。并且,菌株FW10M-9T代表了木糖单胞菌属的新物种,其名称为木糖单胞菌。十一月 提出,型号为FW10M-9T(= KACC 19331T = NBRC 113053T)。分别。根据表型和基因型数据,建议菌株2DFWM-2T代表乳球菌属的一个新物种,其名称为Protaetiae sp.。十一月 提出,类型应变为2DFWM-2T(= KACC 19320T = NBRC 113069T)。并且,菌株FW10M-9T代表了木糖单胞菌属的新物种,其名称为木糖单胞菌。十一月 提出,型号为FW10M-9T(= KACC 19331T = NBRC 113053T)。分别。根据表型和基因型数据,建议菌株2DFWM-2T代表乳球菌属的一个新物种,其名称为Protaetiae sp.。十一月 提出,类型应变为2DFWM-2T(= KACC 19320T = NBRC 113069T)。并且,菌株FW10M-9T代表了木糖单胞菌属的新物种,其名称为木糖单胞菌。十一月 提出,型号为FW10M-9T(= KACC 19331T = NBRC 113053T)。其名称为Xylanimonas protaetiae sp。十一月 提出,型号为FW10M-9T(= KACC 19331T = NBRC 113053T)。其名称为Xylanimonas protaetiae sp。十一月 提出,型号为FW10M-9T(= KACC 19331T = NBRC 113053T)。
更新日期:2020-04-18
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