We studied the levels of serum and lymph cytokines involved in the pathogenesis of BC. BC was induced by injection of N-methyl-N-nitrosourea to Wistar rats. The animals underwent surgery, or received polychemotherapy (cyclophosphamide, methotrexate, and 5-fluorouracil), or surgical treatment was combined with polychemotherapy; in a special group, Panagen (fragmented DNA) was added to polychemotherapy. Cytokine concentration in the lymph was measured using Bio-Plex Pro Rat Cytokoness 24-Plex Assay test system (Bio-Rad). In rats with BC, the content of most studied cytokines (IL-1β, IL-2, IL-4, IL-6, IL-7, IL-12, IL-13, MIP-1α, MIP-3α, RANTES, TNFα, and MCP-1) in the lymph and blood was significantly higher, while the content of IL-10 and GRO/KC was lower than in intact animals. Surgical resection of the tumor led to a significant decrease in the content of both pro- and anti-inflammatory cytokines in the lymph. Polychemotherapy led to a significant decrease in the content of IL-1β, IL-4, IL-6, IL-7, MIP-1α, MIP-3α, and RANTES in the serum and lymph. Comparison of the cytokine content in the serum and lymph of operated animals after polychemotherapy with and without Panagen showed that the content of most cytokines (IL-5, IL-6, IL-7, IL-10, IL-13, IL-17A , IL-18, GRO/KC, IFNγ, and MIP-3α) was higher after Panagen administration.

中文翻译：

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淋巴和血清的细胞因子作为 Wistar 大鼠化学诱导乳腺癌肿瘤发生和治疗效率的标志物

我们研究了参与 BC 发病机制的血清和淋巴细胞因子的水平。通过向 Wistar 大鼠注射 N-甲基-N-亚硝基脲诱导 BC。动物接受手术，或接受综合化疗（环磷酰胺、甲氨蝶呤和5-氟尿嘧啶），或手术治疗联合综合化疗；在一个特殊的群体中，Panagen（DNA 片段化）被添加到多化学疗法中。使用 Bio-Plex Pro Rat Cytokoness 24-Plex Assay 测试系统 (Bio-Rad) 测量淋巴中的细胞因子浓度。在 BC 大鼠中，研究最多的细胞因子（IL-1β、IL-2、IL-4、IL-6、IL-7、IL-12、IL-13、MIP-1α、MIP-3α、RANTES、淋巴和血液中的 TNFα 和 MCP-1) 显着升高，而 IL-10 和 GRO/KC 的含量低于完整动物。手术切除肿瘤导致淋巴中促炎和抗炎细胞因子的含量显着降低。综合化疗导致血清和淋巴液中 IL-1β、IL-4、IL-6、IL-7、MIP-1α、MIP-3α 和 RANTES 的含量显着降低。有无 Panagen 多化疗后手术动物血清和淋巴中细胞因子含量的比较表明，大多数细胞因子（IL-5、IL-6、IL-7、IL-10、IL-13、IL-17A Panagen 给药后，IL-18、GRO/KC、IFNγ 和 MIP-3α) 更高。