Transcription profiles of genes of local immune response were determined in the vagina of women with bacterial vaginosis, aerobic vaginitis, and vulvovaginal candidosis for detection of the most specific immune markers for these vaginal infections. Laboratory diagnosis of the vaginal infections was performed microscopically; the inflammatory reaction in the vagina (leukorrhea) was defined as the presence of >10 white blood cells per field of view. Transcription profiles of IL1b , IL10 , IL18 , TNFα , TLR4 , GATA3 , and CD68 were determined using reverse-transcription quantitative real-time PCR. The strongest predictors of aerobic vaginitis were increased levels of IL1b and IL10 mRNA. Bacterial vaginosis was strongly associated with reduced levels of IL18 and GATA3 mRNA. Increased levels of IL1b and TLR4 transcripts showed significant discriminatory power for vulvovaginal candidosis and leukorrhea. The results of this study suggest differential expression of local immune response genes in the vagina of women with different vaginal infections. Detection of specific immune markers in the vagina using reverse-transcriptase PCR could supplement PCR detection of abnormal vaginal microflora for the diagnosis of vaginal infections.

中文翻译：

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阴道局部免疫反应基因的差异表达：对阴道感染诊断的意义

在患有细菌性阴道病、需氧性阴道炎和外阴阴道念珠菌病的女性的阴道中确定了局部免疫反应基因的转录谱，以检测这些阴道感染的最特异性免疫标志物。阴道感染的实验室诊断是在显微镜下进行的；阴道中的炎症反应（白带）被定义为每个视野中存在 >10 个白细胞。IL1b、IL10、IL18、TNFα、TLR4、GATA3 和 CD68 的转录谱使用逆转录定量实时 PCR 确定。需氧性阴道炎的最强预测因子是 IL1b 和 IL10 mRNA 水平升高。细菌性阴道病与 IL18 和 GATA3 mRNA 水平降低密切相关。增加水平的 IL1b 和 TLR4 转录物显示出对外阴阴道念珠菌病和白带的显着区分能力。这项研究的结果表明，不同阴道感染女性的阴道中局部免疫反应基因的表达存在差异。使用逆转录酶 PCR 检测阴道中的特异性免疫标志物可以补充异常阴道微生物群落的 PCR 检测，用于诊断阴道感染。