Non‐O1/non‐O139 nontoxigenic Vibrio cholerae associated with cholera‐like diarrhea has been reported in Kolkata, India. However, the property involved in the pathogenicity of these strains has remained unclear. The character of 25 non‐O1/non‐O139 nontoxigenic V. cholerae isolated during 8 years from 2007 to 2014 in Kolkata was examined. Determination of the serogroup showed that the serogroups O6, O10, O35, O36, O39, and O70 were represented by two strains in each serogroup, and the remaining isolates belonged to different serogroups. To clarify the character of antibiotic resistance of these isolates, an antibiotic resistance test and the gene analysis were performed. According to antimicrobial drug susceptibility testing, 13 strains were classified as drug resistant. Among them, 10 strains were quinolone resistant and 6 of the 13 strains were resistant to more than three antibiotics. To define the genetic background of the antibiotic character of these strains, whole‐genome sequences of these strains were determined. From the analysis of these sequences, it becomes clear that all quinolone resistance isolates have mutations in quinolone resistance‐determining regions. Further research on the genome sequence showed that four strains possess Class 1 integrons in their genomes, and that three of the four integrons are found to be located in their genomic islands. These genomic islands are novel types. This indicates that various integrons containing drug resistance genes are spreading among V. cholerae non‐O1/non‐O139 strains through the action of newly generated genomic islands.

中文翻译：

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来自印度加尔各答的霍乱弧菌非O1 / non-O139菌株临床分离株中抗生素抗性编码基因的基因组表征：包含多个抗生素抗性基因的新型基因组岛的产生。

印度加尔各答已有非O1 /非O139非毒性霍乱弧菌与霍乱样腹泻有关的报道。但是，这些菌株的致病性所涉及的性质仍不清楚。25个非O1 /非O139非毒素霍乱弧菌的特征从2007年至2014年在加尔各答的8年中分离出的细菌进行了检查。血清群的测定表明，O6，O10，O35，O36，O39和O70血清群由每个血清群中的两个菌株代表，其余分离株属于不同的血清群。为了阐明这些分离株的抗生素抗性的特征，进行了抗生素抗性测试和基因分析。根据抗菌药物敏感性测试，将13株菌株归为耐药菌。其中，10株对喹诺酮类耐药，13株中的6株对三种以上抗生素具有耐药性。为了确定这些菌株的抗生素特性的遗传背景，确定了这些菌株的全基因组序列。通过对这些序列的分析，显然，所有对喹诺酮耐药的菌株在确定喹诺酮耐药性的区域都有突变。对基因组序列的进一步研究表明，四个菌株在其基因组中具有1类整合素，并且发现四个整合素中的三个位于其基因组岛中。这些基因岛是新颖的类型。这表明含有耐药基因的各种整合素正在霍乱弧菌的非O1 /非O139菌株通过新产生的基因岛的作用而产生。