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An ex-situ and in vitro approach towards the bioremediation of carcinogenic hexavalent chromium.
Preparative Biochemistry & Biotechnology ( IF 2.0 ) Pub Date : 2020-04-17 , DOI: 10.1080/10826068.2020.1755868
Neethu Kamarudheen 1 , Sona P Chacko 1 , Catherin A George 1 , Rakhi Chettiparambil Somachandran 1 , K V Bhaskara Rao 1
Affiliation  

Chromium, ranking the second most among toxic heavy metal pollutants in the world, causing respiratory, cardiovascular and renal problems in human beings is under study herein. We examined the biological remediation of the carcinogenic Cr (VI) polluted soils by indigenous yeast isolates. The total element analysis of the treated sample was determined by Energy Dispersion X-ray Micro Analysis (EDXMA). The sample under study was observed to have a high concentration of 458.29 mgKg−1 Cr (VI), determined by Atomic Absorption Spectroscopy (AAS) and DPC analysis. The most tolerant isolate designated as CSR was used for in vitro and ex-situ bioremediation studies of Cr (VI). The isolate achieved significant bioremediation of 86% in vitro and 75.12% in ex-situ method. The optimal conditions for in vitro bioremediation were found to be 28 °C and a pH of 6. The ITS1, 5.8S rRNA and D1, D2 domain of LSU rRNA gene characterization of the isolate CSR illustrated that it belongs to Ustilago genera. The isolate was deposited in NCBI GenBank as Ustilago sp. CSR (KY284846). Although, Ustilago is generally a pathogenic fungus, our study opens up the scope of using Ustilago spp. for bioremediation of the carcinogenic heavy metal Chromium.



中文翻译:

用于致癌六价铬生物修复的异位和体外方法。

本文正在研究在人类有毒的重金属污染物中排名第二的铬,它会引起人类的呼吸,心血管和肾脏问题。我们检查了由本地酵母菌对致癌的六价铬污染土壤的生物修复。经处理的样品的总元素分析通过能量色散X射线显微分析(EDXMA)确定。通过原子吸收光谱法(AAS)和DPC分析测定,观察到的样品的高浓度为458.29 mgKg -1 Cr(VI)。指定为CSR最宽容分离物用于体外易地的Cr(VI)的生物修复研究。该分离物在体外实现了86%的显着生物修复和75.12%易地方法。发现体外生物修复的最佳条件是28°C,pH为6。分离的CSR的LSU rRNA基因的ITS1、5.8S rRNA和D1,D2结构域表明,它属于Ustilago属。分离株以Ustilago sp。保藏在NCBI GenBank中CSR(KY284846)。尽管Ustilago通常是一种致病真菌,但我们的研究开辟了使用Ustilago spp的范围。用于致癌重金属铬的生物修复。

更新日期:2020-04-17
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