Bombyx mori nucleopolyhedrovirus (BmNPV) is a DNA virus that causes huge losses to the silkworm industry but the piRNA responses during BmNPV infection in the silkworm remain uninvestigated. Here, silkworm piRNA profiles of uninfected and BmNPV-infected fat body and midgut were determined by high-through sequencing in the early stages of BmNPV infection. A total of 2675 and 3396 genome-derived piRNAs were identified from fat body and midgut, respectively. These genome-derived piRNAs mainly originated from unannotated instead of transposon regions in the silkworm genome. In total, 572 piRNAs were associated with 280 putative target genes in fat body and 805 piRNAs with 380 target genes in midgut. Compared to uninfected tissues, 322 and 129 piRNAs were significantly up-regulated in BmNPV-infected fat body and midgut, respectively. In addition, 276 and 117 piRNAs were significantly down-regulated. Moreover, differentially expressed (DE) piRNAs during BmNPV infection differed significantly between fat body and midgut. Putative DE piRNA-targeted genes were associated with "response to stimulus" and "environmental information processing" in fat body after infection with BmNPV, which may indicate an active piRNA response to BmNPV infection in fat body. This study may lay the foundation for future research of the potential roles of the piRNA pathway and specific piRNAs in BmNPV pathogenesis. This article is protected by copyright. All rights reserved.

中文翻译：

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家蚕脂肪体和中肠对 BmNPV 感染的 piRNA 反应

家蚕核多角体病毒 (BmNPV) 是一种 DNA 病毒，给家蚕业造成巨大损失，但家蚕中 BmNPV 感染期间的 piRNA 反应仍未得到研究。在这里，在 BmNPV 感染的早期阶段，通过高通量测序确定了未感染和 BmNPV 感染的脂肪体和中肠的家蚕 piRNA 谱。从脂肪体和中肠中分别鉴定出 2675 和 3396 个基因组衍生的 piRNA。这些基因组衍生的 piRNA 主要来源于家蚕基因组中未注释的而不是转座子区域。总共有572个piRNA与脂肪体中的280个推定靶基因相关，805个piRNA与中肠中的380个靶基因相关。与未感染的组织相比，322 和 129 piRNA 在 BmNPV 感染的脂肪体和中肠中分别显着上调。此外，276 和 117 piRNA 显着下调。此外，BmNPV 感染期间差异表达 (DE) piRNA 在脂肪体和中肠之间存在显着差异。假定DE piRNA靶向基因与BmNPV感染后脂肪体的“刺激反应”和“环境信息处理”相关，这可能表明脂肪体中对BmNPV感染的piRNA反应活跃。本研究可能为未来研究 piRNA 通路和特定 piRNA 在 BmNPV 发病机制中的潜在作用奠定基础。本文受版权保护。版权所有。假定DE piRNA靶向基因与BmNPV感染后脂肪体的“刺激反应”和“环境信息处理”相关，这可能表明脂肪体中对BmNPV感染的piRNA反应活跃。本研究可能为未来研究 piRNA 通路和特定 piRNA 在 BmNPV 发病机制中的潜在作用奠定基础。本文受版权保护。版权所有。假定DE piRNA靶向基因与BmNPV感染后脂肪体的“刺激反应”和“环境信息处理”相关，这可能表明脂肪体中对BmNPV感染的piRNA反应活跃。本研究可能为未来研究 piRNA 通路和特定 piRNA 在 BmNPV 发病机制中的潜在作用奠定基础。本文受版权保护。版权所有。