Abstract This study aims the synthesis of a novel functionalized magnetic nanocarrier based on xanthan gum biopolymer. Glycidyl methacrylate was grafted on xanthan gum chains by radical polymerization reaction using two types of initiators: ammonium persulfate and benzoyl peroxide. Characterization studies of the magnetic nanocarrier were performed using several instruments such as Fourier Transform Infrared Spectroscopy, Scanning Electron Microscopy, Energy-Dispersive-X-Ray Spectroscopy, X-Ray Diffraction Spectroscopy, Transmission Electron Microscopy, Mossbauer Spectroscopy, and Vibrating Sample Magnetometer. According to the Vibrating Sample Magnetometer data and Mossbauer analysis, Fe atoms were incorporated successfully in the polymer chains in Fe3+ state and magnetic nanocarrier has super-paramagnetic behavior, respectively. Epoxy groups on magnetic nanoparticles were converted to carboxylic acid groups using iminodiacetic acid and then tested to usability as a carrier for immobilization of proteins such as albumin, lipase and cytochrome c. The adsorption of albumin and lipase on magnetic nanoparticles were pH-dependent while cytochrome c was immobilized in a wide range of pH value. The calculated maximum experimental immobilization capacity of magnetic nanoparticles was 65.10 mg g−1, 62.0 mg g−1 and 188.0 mg g−1 for albumin, lipase and cytochrome c, respectively. Experimental data fitted to Langmuir isotherm better than Freundlich. The rate of cytochrome c adsorption followed the pseudo-second-order kinetic. Results showed that the functionalized magnetic nanoparticles can be effectively used as a carrier for protein separations, especially for cytochrome c. Moreover, the functionalized magnetic nanocarrier had high affinity to Cytochrome c protein even in multiple protein systems.

中文翻译：

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一种新型功能化磁性纳米生物复合材料作为蛋白质吸附载体的制备

摘要 本研究旨在合成一种基于黄原胶生物聚合物的新型功能化磁性纳米载体。使用两种类型的引发剂：过硫酸铵和过氧化苯甲酰，通过自由基聚合反应将甲基丙烯酸缩水甘油酯接枝在黄原胶链上。使用多种仪器对磁性纳米载体进行表征研究，例如傅里叶变换红外光谱、扫描电子显微镜、能量色散 X 射线光谱、X 射线衍射光谱、透射电子显微镜、穆斯堡尔光谱和振动样品磁强计。根据振动样品磁力计数据和 Mossbauer 分析，Fe 原子以 Fe3+ 状态成功掺入聚合物链中，磁性纳米载体分别具有超顺磁行为。使用亚氨基二乙酸将磁性纳米颗粒上的环氧基转化为羧酸基团，然后测试其作为固定蛋白质（如白蛋白、脂肪酶和细胞色素 c）的载体的可用性。白蛋白和脂肪酶在磁性纳米颗粒上的吸附是 pH 依赖性的，而细胞色素 c 固定在很宽的 pH 值范围内。计算出的磁性纳米粒子的最大实验固定容量分别为 65.10 mg g-1、62.0 mg g-1 和 188.0 mg g-1，分别用于白蛋白、脂肪酶和细胞色素 c。符合 Langmuir 等温线的实验数据比 Freundlich 更好。细胞色素 c 的吸附速率遵循伪二级动力学。结果表明，功能化的磁性纳米粒子可以有效地用作蛋白质分离的载体，尤其是细胞色素 c。