Background: Ischemia/reperfusion can cause injury to tissues and compromise functionality of organs due to inflammatory processes. Significantly, development of these effects in kidney tissue has been a challenging issue that leads to acute renal injury. In this study, anti-inflammatory, anti-oxidative, and protective features of dapsone on kidney ischemia/reperfusion injury were investigated.Material and methods: Renal ischemia was induced in rats by bilateral renal arteries clamping for 45 min followed by 24 h reperfusion phase. The effects of different doses of dapsone (1, 3, 10 mg/kg) on ischemia/reperfusion injury in kidney tissue were investigated by targeting BUN, Creatinine, LDH, MDA, MPO, IL-1β, TNF-α, and NFκB. In addition histopathological examination was performed by H&E staining method.Results and discussion: Comparing the findings of this study showed significant reduction in BUN and LDH in 10 mg/kg dapsone received groups, and Cr, MDA, and MPO in 3 mg/kg dapsone received groups. The serum level of TNF-α was significantly decreased with both doses of 3 and 10 mg/kg dapsone. The same results were observed in the serum level of IL-1β and NFκB. Besides, remarkable improvement in histological damages was also observed with dapsone treatment.Conclusion: These results support the hypothesis that the positive effects of dapsone on the renal ischemia/reperfusion injury are mediated by modulating inflammatory cascades.

中文翻译：

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氨苯砜对大鼠肾脏缺血再灌注损伤的保护作用。

背景：缺血/再灌注会由于炎症过程而导致组织损伤并损害器官功能。值得注意的是，在肾脏组织中这些作用的发展一直是导致急性肾损伤的具有挑战性的问题。本研究探讨了氨苯砜对肾脏缺血/再灌注损伤的抗炎，抗氧化和保护作用。材料与方法：大鼠双侧肾动脉夹闭45min，再灌注24h，诱发大鼠肾脏缺血。通过靶向BUN，肌酐，LDH，MDA，MPO，IL-1β，TNF-α和NFκB，研究了不同剂量的氨苯砜（1、3、10 mg / kg）对肾脏组织缺血/再灌注损伤的影响。另外，通过H＆E染色法进行了组织病理学检查。结果与讨论：比较本研究的结果，发现接受氨苯砜10 mg / kg组的BUN和LDH显着降低，接受氨苯砜3 mg / kg组中的Cr，MDA和MPO显着降低。剂量为3和10 mg / kg氨苯砜的患者，血清TNF-α的水平均明显降低。血清IL-1β和NFκB水平也观察到相同的结果。此外，氨苯砜治疗还可以观察到组织学损伤的明显改善。结论：这些结果支持假说氨苯砜对肾脏缺血/再灌注损伤的积极作用是通过调节炎症级联反应介导的。剂量为3和10 mg / kg氨苯砜的患者，血清TNF-α的水平均明显降低。血清IL-1β和NFκB水平也观察到相同的结果。此外，氨苯砜治疗还可以观察到组织学损伤的明显改善。结论：这些结果支持假说氨苯砜对肾脏缺血/再灌注损伤的积极作用是通过调节炎症级联反应介导的。剂量为3和10 mg / kg氨苯砜的患者，血清TNF-α的水平均明显降低。血清IL-1β和NFκB水平也观察到相同的结果。此外，氨苯砜治疗还可以观察到组织学损伤的明显改善。结论：这些结果支持假说氨苯砜对肾脏缺血/再灌注损伤的积极作用是通过调节炎症级联反应介导的。