Background:

Osteoarthritis (OA) in the temporomandibular joint (TMJ) in the TMJ (TMJ-OA) is difficult to treat, and new alternative treatments are needed. Recently, adipose-derived stem cells (ASCs) have been introduced as a promising cell source because of their anti-inflammatory effects. However, the cost and availability of these cells limited broader applications of stem cell therapy. Thus, Thus, stromal vascular fraction (SVF) containing sufficient amount of ASCs at low cost can be an alternative. In this study, we aimed to demonstrate the use of uncultured, optimally isolated SVF for the treatment of TMJ-OA.

Methods:

First, we optimized the method of isolation to harvest high-quality SVFs with a large yield of ASCs. Then, we analyzed the quantity of ASCs in the SVF and performed characterization of stem cell homology. Subsequently, to evaluate the anti-inflammatory effect of high-quality SVF, an in vitro study was performed to assess the expression patterns of inflammatory cytokines including prostaglandin E₂ (PGE₂), IL-6, and CXCL8/IL-8, COX2, TNF, IFN, CCL2/MCP-1 and CCL5/RANTES in co-culture with synoviocytes derived from the synovial fluid in the TMJ-OA patients.

Results:

The SVF containing approximately 32% ASCs was isolated via the our optimized isolation method. The SVF significantly down-regulated certain inflammatory cytokines such as PGE2, CXCL8/IL-8 in TMJ-OA tissue-derived synoviocytes.

Conclusion:

Although further study is needed, our study suggests that transplantation of adipose tissue-derived SVF cells might be a feasible and a novel therapeutic option for TMJ-OA in the future.

中文翻译：

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脂肪基质血管分数对骨关节炎颞下颌关节滑膜细胞的抗炎作用。

背景：

颞下颌关节（TMJ-OA）的颞下颌关节（TMJ）中的骨关节炎（OA）难以治疗，因此需要新的替代疗法。近来，由于脂肪干细胞（ASC）具有抗炎作用，因此已被用作有前途的细胞来源。但是，这些细胞的成本和可用性限制了干细胞疗法的广泛应用。因此，因此，包含低成本的足量ASC的基质血管部分（SVF）可以作为替代方案。在这项研究中，我们旨在证明未培养的，最佳分离的SVF在治疗TMJ-OA中的用途。

方法：

首先，我们优化了分离方法，以收获具有大量ASC的高质量SVF。然后，我们分析了SVF中ASC的数量，并对干细胞同源性进行了表征。随后，为了评估高质量SVF的抗炎作用，进行了一项体外研究，以评估包括前列腺素E ₂（PGE ₂），IL-6和CXCL8 / IL-8，COX2在内的炎性细胞因子的表达模式。，TNF，IFN，CCL2 / MCP-1和CCL5 / RANTES与来自TMJ-OA患者滑膜液的滑膜细胞共培养。

结果：

含有约32％ASC的SVF通过我们优化的分离方法进行了分离。SVF显着下调了TMJ-OA组织来源的滑膜细胞中某些炎症细胞因子，例如PGE2，CXCL8 / IL-8。

结论：

尽管还需要进一步的研究，但我们的研究表明，脂肪组织来源的SVF细胞的移植可能是未来TMJ-OA的可行方法和新的治疗选择。