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Analysis of P78: A Novel Cytoplasmic Membrane-Associated Protein Encoded on Chromosome 19q13.3 in Glioma Specimens.
Journal of Molecular Neuroscience ( IF 2.8 ) Pub Date : 2020-05-04 , DOI: 10.1007/s12031-020-01562-3
Bruce M Frankel 1 , David Cachia 1 , Sunil J Patel 1 , Arabinda Das 1
Affiliation  

Allelic losses of the q13.3 region of chromosome 19 have been documented in all major types of diffuse gliomas, strongly suggesting the presence of a 19q13.3 tumor suppressor gene responsible for these malignancies. The P78 gene precisely maps to 19q13.3, the glioma candidate region, and encodes a recently identified novel protein (P78). The purpose of this study was to determine P78 protein expression in gliomas. Serial analysis of gene expression (SAGE) reveals P78 mRNA expression to be significantly reduced in high-grade gliomas such as glioblastoma (GB), as compared with the low-grade tumors including astrocytomas, oligodendrogliomas, and ependymomas. We observed the distribution of staining of P78 protein was concentrated on the cell membranes of the luminal epithelial cells, not cytoplasm. In contrast, the pre-immune serum controls demonstrated no staining. These results demonstrate that P78 protein is highly expressed in the cytoplasmic membranes of low but not high-grade astrocytomas, and correlates with grade of malignancy. In these double immunostaining experiments, the anti-Map-2 and anti-NeuN antibodies did not stain round cells that were stained with the anti-P78 carboxyl-terminal peptide antibodies, demonstrating that these round cells were not neurons, and likely protoplasmic astrocytes. Current results also suggest that the astrocytes stained with the anti-P78 carboxyl-terminal peptide antibody are likely protoplasmic astrocytes. We also observed preincubation of anti-P78 carboxyl-terminal antibodies with immunizing peptides abolished immunostaining in gliomas. These results suggest a role for the P78 protein in the process of abnormal growth in glial tumors.

中文翻译:

P78的分析:胶质瘤标本中的染色体19q13.3上编码的新型细胞质膜相关蛋白。

在所有主要类型的弥漫性神经胶质瘤中均已记录了19号染色体q13.3区的等位基因缺失,强烈提示存在导致这些恶性肿瘤的19q13.3肿瘤抑制基因。P 78基因精确地映射到神经胶质瘤候选区域19q13.3,并编码最近鉴定的新蛋白(P 78)。这项研究的目的是确定神经胶质瘤中P 78蛋白的表达。基因表达的序列分析(SAGE)显示,与星形胶质瘤,少突胶质细胞瘤和室管膜瘤等低度肿瘤相比,高级别神经胶质瘤(例如胶质母细胞瘤(GB))中P 78 mRNA表达显着降低。我们观察到P 78染色的分布蛋白质集中在腔上皮细胞的细胞膜上,而不是细胞质上。相反,免疫前血清对照显示无染色。这些结果表明,P 78蛋白在低度而非高度星形细胞瘤的细胞质膜中高表达,并且与恶性程度相关。在这些双重免疫染色实验中,抗Map-2和抗NeuN抗体未染色被抗P 78羧基末端肽抗体染色的圆形细胞,表明这些圆形细胞不是神经元,可能不是原生质星形胶质细胞。目前的结果还表明,星形胶质细胞被抗P 78染色羧基末端肽抗体可能是原生质星形胶质细胞。我们还观察到抗-P 78羧基末端抗体与免疫肽的预孵育消除了神经胶质瘤中的免疫染色。这些结果表明P 78蛋白在神经胶质瘤异常生长过程中的作用。
更新日期:2020-05-04
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