Menispermum dauricum is widely used to treat respiratory inflammation, including laryngopharyngitis, tonsillitis, tracheitis, and bronchitis. Total alkaloids isolated from M. dauricum have shown a variety of beneficial bioactivities. However, available data on the effects of M. dauricum total alkaloids against allergic asthma has not been reported. In present study, the protective effect of M. dauricum total alkaloids was evaluated by using an ovalbumin-induced in vivo model of asthma. The asthma model was prepared by sensitizing and challenging mice with ovalbumin, and M. dauricum total alkaloids (100, 200, and 400 mg/kg) were administrated to asthmatic mice by gavage. Histopathological analysis of pulmonary changes was detected by hematoxylin and eosin, and periodic acid-schiff staining. Inflammatory cell counts were determined in bronchoalveolar lavage fluid. Total immunoglobulin E and ovalbumin-specific immunoglobulin E levels in serum, and T-helper 2 cytokines and chemokine levels in bronchoalveolar lavage fluid were detected by an ELISA. Histological results demonstrated that M. dauricum total alkaloids significantly attenuated pulmonary inflammation in asthmatic mice. M. dauricum total alkaloid treatment exhibited marked effects on asthmatic mice in reducing inflammatory cell counts, decreasing interleukin-4, interleukin-5, and interleukin-13 concentrations, and downregulating TNF-α and eotaxin levels in bronchoalveolar lavage fluid. In addition, M. dauricum total alkaloids could also inhibit the elevated serum levels of total immunoglobulin E and ovalbumin-specific immunoglobulin E. These findings confirmed that M. dauricum total alkaloids could suppress airway inflammation in ovalbumin-induced asthma through regulating the T-helper 2 response and chemokine level. M. dauricum total alkaloids may be a potential ethnopharmacological agent for asthmatic patients.

中文翻译：

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黄芩总生物碱对哮喘小鼠气道炎症的保护作用

Menispermum dauricum 广泛用于治疗呼吸道炎症，包括喉咽炎、扁桃体炎、气管炎和支气管炎。从 M. dauricum 中分离出的总生物碱显示出多种有益的生物活性。然而，尚无关于 M. dauricum 总生物碱对过敏性哮喘影响的可用数据报告。在本研究中，通过使用卵清蛋白诱导的哮喘体内模型评估了金缕梅总生物碱的保护作用。通过用卵清蛋白致敏和激发小鼠制备哮喘模型，并通过管饲法给哮喘小鼠施用大金黄总生物碱（100、200和400mg/kg）。通过苏木精和伊红以及高碘酸-希夫染色检测肺部变化的组织病理学分析。在支气管肺泡灌洗液中测定炎性细胞计数。通过ELISA检测血清中的总免疫球蛋白E和卵清蛋白特异性免疫球蛋白E水平，以及支气管肺泡灌洗液中的T辅助2细胞因子和趋化因子水平。组织学结果表明，M. dauricum 总生物碱显着减轻哮喘小鼠的肺部炎症。M. dauricum 总生物碱治疗对哮喘小鼠表现出显着效果，包括减少炎症细胞计数、降低白细胞介素 4、白细胞介素 5 和白细胞介素 13 浓度，以及下调支气管肺泡灌洗液中的 TNF-α 和嗜酸性粒细胞趋化因子水平。此外，M. dauricum 总生物碱还可以抑制血清总免疫球蛋白 E 和卵清蛋白特异性免疫球蛋白 E 的升高。这些发现证实了 M. dauricum 总生物碱。黄芩总生物碱可通过调节 T-helper 2 反应和趋化因子水平抑制卵清蛋白诱导的哮喘气道炎症。M. dauricum 总生物碱可能是哮喘患者的潜在民族药理学药物。