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Identifications, Characteristics, and Expression Patterns of Small Heat Shock Protein Genes in a Major Mulberry Pest, Glyphodes pyloalis (Lepidoptera: Pyralidae).
Journal of Insect Science ( IF 2.1 ) Pub Date : 2020-05-01 , DOI: 10.1093/jisesa/ieaa029
Jie Chu 1 , De-Lei Jiang 1 , Meng-Wen Yan 1 , Yi-Jiangcheng Li 1 , Jun Wang 1, 2 , Fu-An Wu 1, 2 , Sheng Sheng 1, 2
Affiliation  

Six candidate sHSP genes were identified from the Glyphodes pyloalis transcriptome. All sHSP genes included full-length open reading frames and shared high similarity with the sequences of other lepidopteran species. These sHSP genes encoded 175-191 amino acid residues, and the predicted proteins had a molecular weight from 19.5 to 21.8 kDa. All GpsHSPs were expressed at lower levels at larval stages. All GpsHSPs were expressed at higher levels at diapaused, prepupal, or pupal stages, suggesting that sHSPs may be involved in metamorphosis in G. pyloalis. In addition to the developmental stage, extreme temperatures can induce variations in the expression of sHSPs genes. All GpsHSPs were significantly upregulated in larvae following exposure to heat shock, except GpHSP21.4 which downregulated at 4 h following exposure to the cold shock treatment. Furthermore, Starvation influenced the expression patterns of GpsHSPs as a function of the duration of food deprivation. Four GpsHSPs increased their expression with time of starvation until reaching to the peak level at 6 d of starvation. Finally, parasitism by the endoparasitoid Aulacocentrum confusum He et van Achterberg (Hymenoptera: Braconidae)-induced fluctuations in the expression of all GpsHSPs, and the expression varied with time after parasitization. Our results from this study strongly suggest functional differentiation within the sHSPs subfamily in G. pyloalis. The present study would provide further insight into the roles of sHSPs in G. pyloalis and novel avenues for promoting integrated management of this pest.

中文翻译:

鉴定,特征和主要桑树害虫小翅目Glyphodes pyloalis(鳞翅目:Pyralidae)中小热激蛋白基因的表达模式。

从Glyphodes pyloalis转录组中鉴定出六个候选sHSP基因。所有sHSP基因均包含全长开放阅读框,并且与其他鳞翅目物种的序列具有高度相似性。这些sHSP基因编码175-191个氨基酸残基,预测的蛋白质分子量为19.5至21.8 kDa。在幼虫阶段,所有GpsHSPs均以较低的水平表达。所有的GpsHSPs在滞育期,椎前期或p期均以较高的水平表达,表明sHSPs可能参与了幽门螺杆菌的变态。除了发育阶段外,极端温度还可以诱导sHSPs基因表达的变化。暴露于热休克后,幼虫中的所有GpsHSPs均显着上调,除了GpHSP21.4暴露于冷激处理后4小时下调。此外,饥饿影响了GpsHSPs的表达方式,与食物匮乏的持续时间有关。四个GpsHSPs随饥饿时间增加其表达,直至在饥饿6天达到峰值。最后,内寄生寄生虫共生寄生虫He et van Achterberg(膜翅目:Braconidae)引起的寄生效应导致所有GpsHSPs表达的波动,并且寄生后该表达随时间变化。我们从这项研究中得出的结果有力地表明,在幽门螺杆菌的sHSPs亚家族中存在功能分化。本研究将进一步了解sHSPs在幽门螺杆菌中的作用,以及促进该病虫害综合治理的新途径。四个GpsHSP随饥饿时间增加其表达,直至在饥饿6天达到峰值。最后,内寄生寄生虫共生的寄生虫He et van Achterberg(膜翅目:Braconidae)引起的所有GpsHSPs表达的波动,并且寄生后该表达随时间变化。我们从这项研究中得出的结果有力地表明,在幽门螺杆菌的sHSPs亚家族中存在功能分化。本研究将进一步了解sHSPs在幽门螺杆菌中的作用,以及促进该病虫害综合治理的新途径。四个GpsHSP随饥饿时间增加其表达,直至在饥饿6天达到峰值。最后,内寄生寄生虫共生寄生虫He et van Achterberg(膜翅目:Braconidae)引起的寄生效应导致所有GpsHSPs表达的波动,并且寄生后该表达随时间变化。我们从这项研究中得出的结果有力地表明,在幽门螺杆菌的sHSPs亚家族中存在功能分化。本研究将进一步了解sHSPs在幽门螺杆菌中的作用,以及促进该病虫害综合治理的新途径。Braconidae)诱导所有GpsHSPs表达的波动,并且寄生后表达随时间变化。我们从这项研究中得出的结果有力地表明,在幽门螺杆菌的sHSPs亚家族中存在功能分化。本研究将进一步了解sHSPs在幽门螺杆菌中的作用,以及促进该病虫害综合治理的新途径。Braconidae)诱导所有GpsHSPs表达的波动,并且寄生后表达随时间变化。我们从这项研究中得出的结果有力地表明,在幽门螺杆菌的sHSPs亚家族中存在功能分化。本研究将进一步了解sHSPs在幽门螺杆菌中的作用,以及促进该病虫害综合治理的新途径。
更新日期:2020-05-04
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