Abstract

Carbon nanotubes (CNTs) are useful for extracting chemical compounds due to their properties, such as surface area and the potential for chemical modification. Especially the formation of CNTs with carboxylic acid functional group makes them disperse in water-based samples and have strong interaction forces with cationizable analytes. Based on these features, carboxylic acid functionalized multi-walled CNTs (COOH-MWCNTs) have been used as extraction sorbents. CNT can also be gathered using an external magnet by forming complex with iron oxide (Fe₃O₄) magnetic nanoparticles (MNPs). In this study, COOH-MWCNTs with MNPs were subjected to magnetic solid-phase extraction (mSPE) in order to extract the targeted substances such as diphenhydramine, doxylamine, tramadol, escitalopram, zolpidem, diphenamid, paclobutrazol, hexaconazole, cyproconazole and mepronil from human plasma samples. The following five factors were optimized: (i) the ratio of COOH-MWCNTs to MNPs as a sorbent from 1:1 to 1:4; (ii) sorbent amount starting from 12.5 to 75%; (iii) sample pH tested pH 2 to pH 10 with 1 N hydrochloride and 1 N sodium hydroxide; (iv) agitating time from 0 to 4 min and (v) elution solvent. Limit of detection of 10 targeted substances in human plasma were in the range of 0.1–0.4 mg/L. The recovery of targeted substances (except diphenamid) in human plasma was 73.06–110.28% for intra-day and 83.00–107.70% for inter-day and the precision (relative standard deviation, %) in human plasma was 0.3–13.3% for intra-day and 2.9–15.6% for inter-day. The method was applied to nine authentic biological samples from overdose patients in the emergency room of Chungnam National University Hospital. The performance of mSPE was compared with the liquid–liquid extraction method using ethyl acetate. The results showed that the newly developed method in this study can be used for screening analysis in forensic and clinical toxicology.

中文翻译：

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使用COOH-mMWCNTs从人血浆中磁性固相萃取药物和农药

摘要

碳纳米管（CNT）由于其特性（例如表面积和化学修饰的潜力）可用于提取化合物。特别是具有羧酸官能团的CNT的形成使其分散在水基样品中，并且与可阳离子化的分析物具有很强的相互作用力。基于这些特征，已将羧酸官能化的多壁CNT（COOH-MWCNT）用作萃取吸附剂。CNT也可以使用外部磁体通过与氧化铁（Fe ₃ O ₄磁性纳米粒子（MNP）。在这项研究中，对具有MNP的COOH-MWCNT进行磁性固相萃取（mSPE），以便从人源中提取目标物质，例如苯海拉明，多西拉敏，曲马多，依他普仑，唑吡坦，苯乙酰胺，多效唑，六康唑，环丙康唑和甲腈血浆样品。优化了以下五个因素：（i）COOH-MWCNT与作为吸附剂的MNP的比例为1：1至1：4；（ii）吸附剂的量为12.5％至75％；（iii）用1 N盐酸盐和1 N氢氧化钠将样品的pH值测试为pH 2至pH 10；（iv）从0到4分钟的搅拌时间和（v）洗脱溶剂。人血浆中10种目标物质的检出限为0.1-0.4 mg / L。人血浆中目标物质（苯乙酰胺除外）的日内回收率为73.06–110.28％，日内回收率为83.00–107。日间70％，日间血浆中人血浆的精确度（相对标准偏差，％）为0.3–13.3％，日间为2.9–15.6％。该方法已应用于忠南国立大学医院急诊室中过量患者的九种真实生物样品。将mSPE的性能与使用乙酸乙酯的液-液萃取方法进行了比较。结果表明，本研究中新开发的方法可用于法医和临床毒理学的筛选分析。将mSPE的性能与使用乙酸乙酯的液-液萃取方法进行了比较。结果表明，本研究中新开发的方法可用于法医和临床毒理学的筛选分析。将mSPE的性能与使用乙酸乙酯的液-液萃取方法进行了比较。结果表明，本研究中新开发的方法可用于法医和临床毒理学的筛选分析。