The analysis of blood samples for forensic or clinical intoxication cases is a daily routine in an analytical laboratory. The list of "suspect" drugs of abuse and pharmaceuticals that should be ideally screened is large, so multi-targeted methods for comprehensive detection and quantification is a useful tool in the hands of a toxicologist. In this study the development of an UHPLC-MS-MS method is described for the detection and quantification of 84 drugs and pharmaceuticals in postmortem blood. The target compounds comprise pharmaceutical drugs (antipsychotics, antidepressants etc.), some of the most important groups of drugs of abuse: opiates, cocaine, cannabinoids, amphetamines, benzodiazepines and new psychoactive substances (NPS). Sample pretreatment was studied applying a modified Mini-QuEChERS single step, and the best results were obtained after adding in 200 μL of sample, a mixture of 20 mg MgSO4, 5 mg K2CO3 and 5 mg NaCl together with 600 μL of cold acetonitrile. After centrifugation, the supernatant was collected for direct injection. LC-MS analysis took place on a C18 column with a gradient elution over 17 minutes. The method was found to be selective, and sensitive offering limits of detection (LOD) ranging from 0.01 to 9.07 ng/mL. Validation included evaluation of limit of quantification (LOQ), recovery, carry-over, matrix effect, accuracy and precision of the method. The method performed satisfactory in relation to established bioanalytical criteria and was therefore applied to the analysis of blood obtained postmortem from chronic drug abusers, offering unambiguous identification and quantitative determination of drugs in postmortem blood.

中文翻译：

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UHPLC-MS-MS方法测定血液中84种滥用药物和药物。

在分析实验室中，日常分析法医或临床中毒病例的血样是日常工作。理想情况下，应该筛选的“可疑”滥用药物和药物清单很大，因此，多目标方法进行全面检测和定量分析是毒理学家掌握的有用工具。在这项研究中，描述了一种UHPLC-MS-MS方法的开发，用于检测和定量死后血液中的84种药物和药物。目标化合物包括药物（抗精神病药，抗抑郁药等），一些最重要的滥用药物：阿片剂，可卡因，大麻素，苯丙胺，苯二氮卓类和新的精神活性物质（NPS）。采用改良的Mini-QuEChERS单步法对样品预处理进行了研究，加入200μL样品，20 mg MgSO4、5 mg K2CO3和5 mg NaCl与600μL冷乙腈的混合物后，可获得最佳结果。离心后，收集上清液用于直接注射。LC-MS分析在C18柱上进行，梯度洗脱超过17分钟。发现该方法具有选择性，并且提供的检测限（LOD）敏感范围为0.01至9.07 ng / mL。验证包括评估定量限（LOQ），回收率，残留，基质效应，方法的准确性和精密度。该方法相对于已建立的生物分析标准表现令人满意，因此可用于分析从慢性吸毒者那里获得的死后血液，从而提供了明确的鉴定和定量测定死后血液中药物的方法。