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Engineering and biological assessment of double core nanoplatform for co-delivery of hybrid fluorophores to human melanoma.
Journal of Inorganic Biochemistry ( IF 3.8 ) Pub Date : 2020-05-05 , DOI: 10.1016/j.jinorgbio.2020.111088 Urszula Bazylińska 1 , Dominika Wawrzyńczyk 2 , Anna Szewczyk 3 , Julita Kulbacka 4
Journal of Inorganic Biochemistry ( IF 3.8 ) Pub Date : 2020-05-05 , DOI: 10.1016/j.jinorgbio.2020.111088 Urszula Bazylińska 1 , Dominika Wawrzyńczyk 2 , Anna Szewczyk 3 , Julita Kulbacka 4
Affiliation
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We investigated new development in photodynamic therapy (PDT), aiming at enhanced tumor selectivity and biocompatibility, which included application of a third-generation photosensitizing agent, i.e. xanthene-origin Rose Bengal (RB) co-encapsulated with up-converting NaYF4 nanoparticles (NPs) co-doped with lanthanide ions: Er3+ (2%) and Yb3+ (20%). The hybrid fluorophores were applied as components of double core nanocarriers (NCs) obtained by double (multiple) emulsion solvent evaporation process. Next, to improve the biocompatibility and photodynamic activity, biodegradable polymer: poly(lactide-co-glycolide) - PLGA and non-ionic surfactants with different hydrophobicity: Span 80 and Cremophor A25, were used. After the engineering process, controlled by dynamic light scattering (DLS) measurements, ζ-potential evaluation, transmission electron and atomic force microscopy (TEM and AFM) imaging, as well as optical analysis provided by measurements of the up-conversion emission spectra and luminescence kinetics for encapsulated only NaYF4:Er3+,Yb3+ NPs and co-encapsulated RB + NaYF4:Er3+,Yb3+ molecules, spherical polyester NCs with average size <200 nm, were tested on human melanoma (Me-45 and MeWo) cells and a control human keratinocyte (HaCaT) cell line. The photodynamic action of the investigated NCs was assessed by oxidoreductive potential measurements with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, that corresponds to percentage of the viable cells. Immunofluorescence and the NCs internalization studies were visualized by confocal laser scanning microscopy (CLSM studies). Our results indicated effective photosensitizer delivery into the cancer cells and significant photodynamic efficiency enhanced by the near infrared (NIR)-activation of the encapsulated hybrid cargo in the skin melanoma cells.
中文翻译：
工程和生物学评估的双核纳米平台共同向人类黑色素瘤杂交荧光团的交付。
我们研究了光动力疗法（PDT）的新发展，旨在增强肿瘤的选择性和生物相容性，其中包括应用第三代光敏剂，即与上转换的NaYF4纳米粒子（NPs）共封装的x吨起源的玫瑰红（RB）。 ）与镧系元素离子共掺杂：Er3 +（2％）和Yb3 +（20％）。将杂化荧光团用作通过双（多）乳液溶剂蒸发工艺获得的双核纳米载体（NC）的组分。接下来，为了提高生物相容性和光动力活性，使用了可生物降解的聚合物：聚（丙交酯-乙交酯）-PLGA和具有不同疏水性的非离子表面活性剂：Span 80和Cremophor A25。经过工程处理后，通过动态光散射（DLS）测量，ζ势评估，透射电子和原子力显微镜（TEM和AFM）成像，以及通过仅封装NaYF4：Er3 +，Yb3 + NP和共封装RB + NaYF4：Er3 +的上转换发射光谱和发光动力学测量提供的光学分析，在人黑素瘤（Me-45和MeWo）细胞和对照人角质形成细胞（HaCaT）细胞系上测试了Yb3 +分子（平均尺寸小于200 nm的球形聚酯NCs）。通过使用3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四唑溴甲烷（MTT）测定氧化还原电位来评估所研究NC的光动力作用，该测定相当于活细胞的百分比。通过共聚焦激光扫描显微镜（CLSM研究）可以看到免疫荧光和NCs内在化研究。
更新日期：2020-05-05
中文翻译：
工程和生物学评估的双核纳米平台共同向人类黑色素瘤杂交荧光团的交付。
我们研究了光动力疗法（PDT）的新发展，旨在增强肿瘤的选择性和生物相容性，其中包括应用第三代光敏剂，即与上转换的NaYF4纳米粒子（NPs）共封装的x吨起源的玫瑰红（RB）。 ）与镧系元素离子共掺杂：Er3 +（2％）和Yb3 +（20％）。将杂化荧光团用作通过双（多）乳液溶剂蒸发工艺获得的双核纳米载体（NC）的组分。接下来，为了提高生物相容性和光动力活性，使用了可生物降解的聚合物：聚（丙交酯-乙交酯）-PLGA和具有不同疏水性的非离子表面活性剂：Span 80和Cremophor A25。经过工程处理后，通过动态光散射（DLS）测量，ζ势评估，透射电子和原子力显微镜（TEM和AFM）成像，以及通过仅封装NaYF4：Er3 +，Yb3 + NP和共封装RB + NaYF4：Er3 +的上转换发射光谱和发光动力学测量提供的光学分析，在人黑素瘤（Me-45和MeWo）细胞和对照人角质形成细胞（HaCaT）细胞系上测试了Yb3 +分子（平均尺寸小于200 nm的球形聚酯NCs）。通过使用3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四唑溴甲烷（MTT）测定氧化还原电位来评估所研究NC的光动力作用，该测定相当于活细胞的百分比。通过共聚焦激光扫描显微镜（CLSM研究）可以看到免疫荧光和NCs内在化研究。
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