Abstract This study aimed to develop a simple and effective extraction method for taurine in bovine liver through ultrasonic-assisted traditional boiling. Different operating conditions, including liquid-to-solid ratio (1:3–1:7), ultrasonic time (5–25 min), and ultrasonic power (150–300 W) were optimized through single-factor experiments and response surface methodology. Taurine extraction yield was 6.17 ± 0.17 mg/g when the liquid-to-solid ratio was 1:4 mL/g, ultrasonic time was 12 min, and ultrasonic power was 205 W. The regression coefficient of the model established through Design–Expert software was greater than 99%, and the maximum error from the verification experiments results was less than 2%, indicating good credibility. The time under ultrasonic-assisted extraction was 5–7 times lower than that for traditional boiling. Results of Fourier transform infrared spectroscopy and nuclear magnetic resonance (NMR) showed that the extracted taurine has the absorption peaks of NH2 and S O3H, which are consistent with the infrared absorption peak and NMR of the taurine standard. The proposed ultrasonic-assisted traditional boiling method for taurine extraction from bovine liver could effectively reduce the extraction time and could be applied to industrial production.

中文翻译：

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牛肝中牛磺酸的超声辅助提取纯化

摘要 本研究旨在建立一种简单有效的超声波辅助传统煮沸法提取牛肝中牛磺酸的方法。通过单因素实验和响应面方法优化了不同的操作条件，包括液固比（1:3–1:7）、超声时间（5–25 min）和超声功率（150–300 W） . 当液固比为1:4 mL/g，超声时间为12 min，超声功率为205 W时，牛磺酸提取率为6.17±0.17 mg/g。 通过Design-Expert建立的模型回归系数软件大于99%，验证实验结果最大误差小于2%，可信度较好。超声辅助提取时间比传统煮沸时间低5-7倍。傅里叶变换红外光谱和核磁共振(NMR)结果表明，提取的牛磺酸具有NH2和SO3H的吸收峰，与牛磺酸标准品的红外吸收峰和NMR一致。所提出的超声波辅助传统煮沸法从牛肝中提取牛磺酸，可有效缩短提取时间，可应用于工业化生产。