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Multiple stressor responses are regulated by sirtuins in Mytilus congeners.
Comparative Biochemistry and Physiology A: Molecular & Integrative Physiology ( IF 2.1 ) Pub Date : 2020-05-04 , DOI: 10.1016/j.cbpa.2020.110719 M Christina Vasquez 1 , Daniela A Martinez 1 , Lars Tomanek 1
Comparative Biochemistry and Physiology A: Molecular & Integrative Physiology ( IF 2.1 ) Pub Date : 2020-05-04 , DOI: 10.1016/j.cbpa.2020.110719 M Christina Vasquez 1 , Daniela A Martinez 1 , Lars Tomanek 1
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Understanding physiological tolerances of marine organisms to environmental stress is key to predicting species susceptability under climate change. Along the Pacific Coast of the U.S.A. intertidal mussel congeners (genus Mytilis) vary in their physiological stress tolerances, with the invasive M. galloprovincialis being heat tolerant but vulnerable to hyposalinity while the native M. trossulus is vulnerable to heat stress and tolerant of hyposalinity. Sirtuins, a family of NAD+-dependent deacylases, may influence the environmental stressor tolerances in these mussel congeners. The purpose of our study was to determine the mechanism by which sirtuins may confer differential stress responses in the two mussel congeners. Mussels (N = 6 per species) were acclimated to laboratory conditions in tidal simulators and exposed to sirtuin inhibitors (suramin and nicotinamide). Following inhibition, mussels were exposed to hyposalinity stress (29 ppt) for 6 h followed by aerial heat stress (32 °C) for 6 h after which mussel gill was dissected for proteomic analysis. During sirtuin inhibition we found a reduction of cellular stress response (CSR) proteins (molecular chaperones, antioxidants), which are key to maintaining cellular homeostasis. Moreover, we found differential stress responses between the two species under aerial heat combined with hyposalinity exposure. Three-way interactions (aerial heat, hyposalinity and sirtuin inhibition combined) showed complex interactive effects with sirtuins as potential modulators. Thus, our study suggests that sirtuins are contributing to the species-specific CSR in Mytilus and our multiple-stressor approach provides information used to make predictions regarding climate change effects on these competing species.
中文翻译：
Mytilus同源物中的沉默调节蛋白调节多种应激反应。
了解海洋生物对环境压力的生理耐受性是预测气候变化下物种易感性的关键。在美国太平洋沿岸，潮间贻贝同类物（Mytilis属）的生理压力耐受性各不相同，侵入性的墨西哥省分枝杆菌对热具有耐受性，但易受低盐度的影响，而土生的M. trossulus易受热胁迫和对低盐度的耐受性。Sirtuins是NAD +依赖性脱酰基酶的一个家族，可能会影响这些贻贝同类动物的环境胁迫耐受性。我们的研究目的是确定沉默调节蛋白可能在两个贻贝同类物中赋予不同的应激反应的机制。贻贝（每物种N = 6）在潮汐模拟器中适应实验室条件，并暴露于瑟土因抑制剂（苏拉明和烟酰胺）中。抑制后，贻贝暴露于低盐度胁迫下（29 ppt）6小时，然后暴露于空气热胁迫（32°C）6小时，然后解剖贻贝g进行蛋白质组学分析。在抑制沉默调节蛋白的过程中，我们发现细胞应激反应（CSR）蛋白（分子伴侣，抗氧化剂）减少，这是维持细胞稳态的关键。此外，我们发现在空气加热和低盐度暴露下这两个物种之间的应力响应差异。三向相互作用（空气热，次盐度和抑制瑟土因的抑制作用结合在一起）显示了以瑟土因作为潜在调节剂的复杂相互作用。从而，
更新日期：2020-05-04
中文翻译：
Mytilus同源物中的沉默调节蛋白调节多种应激反应。
了解海洋生物对环境压力的生理耐受性是预测气候变化下物种易感性的关键。在美国太平洋沿岸，潮间贻贝同类物（Mytilis属）的生理压力耐受性各不相同，侵入性的墨西哥省分枝杆菌对热具有耐受性，但易受低盐度的影响，而土生的M. trossulus易受热胁迫和对低盐度的耐受性。Sirtuins是NAD +依赖性脱酰基酶的一个家族，可能会影响这些贻贝同类动物的环境胁迫耐受性。我们的研究目的是确定沉默调节蛋白可能在两个贻贝同类物中赋予不同的应激反应的机制。贻贝（每物种N = 6）在潮汐模拟器中适应实验室条件，并暴露于瑟土因抑制剂（苏拉明和烟酰胺）中。抑制后，贻贝暴露于低盐度胁迫下（29 ppt）6小时，然后暴露于空气热胁迫（32°C）6小时，然后解剖贻贝g进行蛋白质组学分析。在抑制沉默调节蛋白的过程中，我们发现细胞应激反应（CSR）蛋白（分子伴侣，抗氧化剂）减少，这是维持细胞稳态的关键。此外，我们发现在空气加热和低盐度暴露下这两个物种之间的应力响应差异。三向相互作用（空气热，次盐度和抑制瑟土因的抑制作用结合在一起）显示了以瑟土因作为潜在调节剂的复杂相互作用。从而，
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