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Proteoglycans exert a significant effect on human meniscal stiffness through ionic effects.
Clinical Biomechanics ( IF 1.4 ) Pub Date : 2020-05-04 , DOI: 10.1016/j.clinbiomech.2020.105028
Mr Fahd Mahmood 1 , Mr Jon Clarke 2 , Dr Philip Riches 3
Affiliation  

BACKGROUND Proteoglycans contribute to mechanical stiffness in articular cartilage, aiding load transmission. The magnitude of the ionic contribution of proteoglycans to the stiffness of human meniscal tissue has not been established. METHODS Thirty-six discs of human meniscal tissue were placed within a custom confined compression chamber and bathed in three solutions of increasing ionic concentration. Following a 0.3 N preload, at equilibrium, a 10% ramp compressive strain was followed by a 7200 s hold phase. A nonlinear poroviscoelastic model with strain dependent permeability was fitted to resultant stress relaxation curves. All samples were assayed for proteoglycan content. Model parameters were analysed using multivariate analysis of variance whilst proteoglycan content was compared using a univariate analysis of variance model. FINDINGS A significant difference (p < .05) was observed in the value of the Young's modulus (E) between samples tested in deionised water compared to those tested in solutions of high ionic concentration. No differences were observed in the zero-strain permeability or the exponential strain dependent stiffening coefficient. Proteoglycan content was not found to differ with solution; but was found to be significantly increased in the middle meniscal region of both menisci. INTERPRETATION Proteoglycans make a significant ionic contribution to mechanical stiffness of the meniscus, increasing it by 58% in the physiological condition. It is therefore critical that meniscal regeneration strategies attempt to recreate the function of proteoglycans to ensure normal meniscal function.

中文翻译:

蛋白聚糖通过离子作用对人的半月板僵硬起重要作用。

背景技术蛋白聚糖有助于关节软骨的机械刚度,有助于负荷传递。蛋白聚糖对人半月板组织僵硬的离子贡献量尚未确定。方法将36个人类半月板组织的椎间盘置于定制的密闭压缩室内,并浸入三种增加离子浓度的溶液中。在承受0.3 N的预紧力后,在平衡状态下,出现10%的斜率压缩应变,然后保持7200 s。将具有应变依赖渗透率的非线性多孔弹性模型拟合到所得应力松弛曲线。测定所有样品的蛋白聚糖含量。使用多变量方差分析来分析模型参数,同时使用单变量方差分析来比较蛋白聚糖含量。发现在去离子水中测试的样品与在高离子浓度溶液中测试的样品之间的杨氏模量(E)值之间存在显着差异(p <.05)。零应变磁导率或指数应变相关的加劲系数没有发现差异。未发现蛋白聚糖含量随溶液的不同而变化。但发现在两个半月板的中间半月板区域均显着增加。解释蛋白聚糖对半月板的机械刚度起着重要的离子作用,在生理条件下增加了58%。因此,至关重要的是,半月板再生策略试图重新创建蛋白聚糖的功能以确保正常的半月板功能。与在高离子浓度溶液中测试的样品相比,在去离子水中测试的样品之间的杨氏模量(E)值观察到05)。在零应变渗透率或指数应变相关的加劲系数方面未观察到差异。未发现蛋白聚糖含量随溶液的不同而变化。但发现在两个半月板的中间半月板区域均显着增加。解释蛋白聚糖对半月板的机械刚度起了重要的离子作用,在生理条件下增加了58%。因此,至关重要的是,半月板再生策略试图重新创建蛋白聚糖的功能以确保正常的半月板功能。与在高离子浓度溶液中测试的样品相比,在去离子水中测试的样品之间的杨氏模量(E)值观察到05)。在零应变渗透率或指数应变相关的加劲系数方面未观察到差异。未发现蛋白聚糖含量随溶液的不同而变化。但发现在两个半月板的中间半月板区域均显着增加。解释蛋白聚糖对半月板的机械刚度起着重要的离子作用,在生理条件下增加了58%。因此,至关重要的是,半月板再生策略试图重新创建蛋白聚糖的功能以确保正常的半月板功能。与在高离子浓度溶液中测试的样品相比,在去离子水中测试的样品之间的s模量(E)。零应变磁导率或指数应变相关的加劲系数没有发现差异。未发现蛋白聚糖含量随溶液的不同而变化。但发现在两个半月板的中间半月板区域均显着增加。解释蛋白聚糖对半月板的机械刚度起着重要的离子作用,在生理条件下增加了58%。因此,至关重要的是,半月板再生策略试图重新创建蛋白聚糖的功能以确保正常的半月板功能。在去离子水中测试的样品与在高离子浓度溶液中测试的样品之间的比模量(E)。在零应变渗透率或指数应变相关的加劲系数方面未观察到差异。未发现蛋白聚糖含量随溶液的不同而变化。但发现在两个半月板的中间半月板区域均显着增加。解释蛋白聚糖对半月板的机械刚度起着重要的离子作用,在生理条件下增加了58%。因此,至关重要的是,半月板再生策略试图重新创建蛋白聚糖的功能以确保正常的半月板功能。未发现蛋白聚糖含量随溶液的不同而变化。但发现在两个半月板的中间半月板区域均显着增加。解释蛋白聚糖对半月板的机械刚度起着重要的离子作用,在生理条件下增加了58%。因此,至关重要的是,半月板再生策略试图重新创建蛋白聚糖的功能以确保正常的半月板功能。未发现蛋白聚糖含量随溶液的不同而变化。但发现在两个半月板的中间半月板区域均显着增加。解释蛋白聚糖对半月板的机械刚度起了重要的离子作用,在生理条件下增加了58%。因此,至关重要的是,半月板再生策略试图重新创建蛋白聚糖的功能以确保正常的半月板功能。
更新日期:2020-05-04
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