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Anti-allergy effect of mojabanchromanol isolated from Sargassum horneri in bone marrow-derived cultured mast cells
Algal Research ( IF 4.6 ) Pub Date : 2020-05-04 , DOI: 10.1016/j.algal.2020.101898
Hyun-Soo Kim , Eui Jeong Han , Ilekuttige Priyan Shanura Fernando , Kalu Kapuge Asanka Sanjeewa , Thilina Uduwaka Jayawardena , Hak-Ju Kim , Youngheun Jee , Seo-Hee Kang , Jae-Hyuk Jang , Jun-Pil Jang , Kalahe Hewage Iresha Nadeeka Madushani Herath , You-Jin Jeon , Ginnae Ahn

This study was conducted to isolate and identify an active compound possessing anti-allergic property from the Jeju Island inhabiting Sargassum horneri (S. horneri). The ethanol extract of S. horneri (SHE) exhibited an anti-allergic effect via the reduction of β-hexosaminidase release in immunoglobulin E (IgE)/bovine serum albumin (BSA)-stimulated bone marrow-derived cultured mast cells (BMCMCs). SHE was subjected to liquid phase fractionation obtaining the hexane fraction, and this was further purified via ODS column chromatography and prep-C18 HPLC. The isolated compound was identified as mojabanchromanol (MC) by 1H, 13C NMR, and LCMS analysis. Isolated MC showed a higher inhibitory effect on the β-hexosaminidase release than those of SHE without cytotoxicity. Moreover, MC inhibited the mRNA expression levels of allergic cytokines in BMCMCs. This is the first report to inform on the anti-allergic property of MC isolated from S. horneri. In conclusion, SHE, as well as MC, is applicable in the development of functional health food, cosmetics, and pharmaceutical products exhibiting anti-allergic effects.



中文翻译:

mojabanchromanol从分离的抗过敏效果铜藻在骨髓衍生培养的肥大细胞

进行该研究以从居住在Sargassum horneriS . horneri)的济州岛分离并鉴定具有抗过敏特性的活性化合物。S的乙醇提取物。霍尼氏(SHE)通过减少免疫球蛋白E(IgE)/牛血清白蛋白(BSA)刺激的骨髓培养的肥大细胞(BMCMCs)中的β-己糖胺酶释放而表现出抗过敏​​作用。将SHE进行液相分馏,得到己烷馏分,并将其通过ODS柱色谱和制备C18 HPLC进一步纯化。到1 H,13时,分离出的化合物被鉴定为mojabanchromanol(MC)1 H NMR和LCMS分析。分离的MC对β-己糖胺酶释放的抑制作用比没有细胞毒性的SHE具有更高的抑制作用。此外,MC抑制了BMCMC中变应性细胞因子的mRNA表达水平。这是第一个报道分离自S的MC的抗过敏特性的报告。霍内里。总之,SHE和MC均可用于开发具有抗过敏作用的功能性保健食品,化妆品和药品。

更新日期:2020-05-04
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