This study was conducted to isolate and identify an active compound possessing anti-allergic property from the Jeju Island inhabiting Sargassum horneri (S. horneri). The ethanol extract of S. horneri (SHE) exhibited an anti-allergic effect via the reduction of β-hexosaminidase release in immunoglobulin E (IgE)/bovine serum albumin (BSA)-stimulated bone marrow-derived cultured mast cells (BMCMCs). SHE was subjected to liquid phase fractionation obtaining the hexane fraction, and this was further purified via ODS column chromatography and prep-C18 HPLC. The isolated compound was identified as mojabanchromanol (MC) by ¹H, ¹³C NMR, and LCMS analysis. Isolated MC showed a higher inhibitory effect on the β-hexosaminidase release than those of SHE without cytotoxicity. Moreover, MC inhibited the mRNA expression levels of allergic cytokines in BMCMCs. This is the first report to inform on the anti-allergic property of MC isolated from S. horneri. In conclusion, SHE, as well as MC, is applicable in the development of functional health food, cosmetics, and pharmaceutical products exhibiting anti-allergic effects.

进行该研究以从居住在Sargassum horneri（S . horneri）的济州岛分离并鉴定具有抗过敏特性的活性化合物。S的乙醇提取物。霍尼氏（SHE）通过减少免疫球蛋白E（IgE）/牛血清白蛋白（BSA）刺激的骨髓培养的肥大细胞（BMCMCs）中的β-己糖胺酶释放而表现出抗过敏​​作用。将SHE进行液相分馏，得到己烷馏分，并将其通过ODS柱色谱和制备C18 HPLC进一步纯化。到¹ H，^13时，分离出的化合物被鉴定为mojabanchromanol（MC）1 H NMR和LCMS分析。分离的MC对β-己糖胺酶释放的抑制作用比没有细胞毒性的SHE具有更高的抑制作用。此外，MC抑制了BMCMC中变应性细胞因子的mRNA表达水平。这是第一个报道分离自S的MC的抗过敏特性的报告。霍内里。总之，SHE和MC均可用于开发具有抗过敏作用的功能性保健食品，化妆品和药品。