The abundance of miR-1b-3p in the chicken ovary is greater after sexual maturation. In the present study there was assessment of whether a single nucleotine polymorphism (SNP) led to an alteration in expression of reproductive traits. The miR-1b-3p abundance was greatest in ovarian follicles The SNP site of rs737028527 (G > A), located in the 734 bp upstream region of pre-miR-1b-3p, was identified in three different chicken breeds. Results from an association analysis of chicken egg-laying traits indicated the SNP was associated with age at first egg production (AFE) and egg number at 32 and 48 weeks (E32, E48; P < 0.01). Hens with genotype AA had an earlier AFE and greater E32 and E48 than hens with other genotypes. The abundance of mature miR-1b-3p in the hens with the GG genotype was larger than those with the AA genotype (P < 0.01), and the luciferase activity of GG genotype promoter was also greater in birds with the AA than GG genotype (P < 0.05). There was inhibition of the production of the transcription factor bound by the specificity protein 1 (Sp1) as a result of the G-to-A mutation, and the luciferase activity of the GG, but not AA, genotype was markedly increased by Sp1. In conclusion, the SNP, rs737028527 (G> A), affected the abundance of mature miR-1b-3p by Sp1 and was associated with chicken egg-laying traits. Data from the present study allow for an increased understanding of the functions and regulation of miR-1b-3p in ovarian follicle development of hens.

性成熟后，鸡卵巢中miR-1b-3p的丰度更高。在本研究中，评估了单个核苷多态性（SNP）是否导致生殖性状表达的改变。在卵巢卵泡中，miR-1b-3p的丰度最大。在三个不同的鸡品种中，rs737028527的SNP位点（G> A）位于miR-1b-3p的上游734 bp。鸡产卵性状的关联分析结果表明，SNP与首次产卵（AFE）的年龄和32和48周的卵数有关（E32，E48；P<0.01）。基因型为AA的母鸡比其他基因型的母鸡具有更早的AFE和更大的E32和E48。具有GG基因型的母鸡中成熟miR-1b-3p的丰度高于具有AA基因型的母鸡（P <0.01），并且具有AA基因的母鸡中GG基因型启动子的萤光素酶活性也大于GG基因型（P <0.05）。G-to-A突变导致特异性蛋白1（Sp1）结合的转录因子的产生受到抑制，而Sp1显着增加了GG的荧光素酶活性，而AA没有显着提高其基因型。总之，SNP rs737028527（G> A）影响了Sp1对成熟miR-1b-3p的丰度并与产卵性状有关。来自本研究的数据使人们对miR-1b-3p在母鸡卵巢卵泡发育中的功能和调控有了更多的了解。