For the commercial culture of the three‐spot cichlid (Amphilophus trimaculatus), it is important to have available a set of protein and lipid ingredients suitable for feed manufacturing, which allow to optimize the culture at affordable costs. The in vitro digestibility was evaluated with pH‐stat technique, using stomach and intestine multi‐enzymatic extracts from A. trimaculatus juveniles. The digestion of protein ingredients was taken place both in acid and in alkaline conditions, quantifying the total of free amino acids at the end of the hydrolysis, whereas the digestion of lipid ingredients was taken place only in alkaline conditions. The digestive enzymes of this species showed a better affinity to raw materials of animal origin such as beef meal, fish meal and premium chicken meal, which presented better digestibility. The best lipid ingredient that could be used is the fish oil. However, it is possible to use ingredients from the plant sources, such as soy or cereals, and vegetable oils such as olive, corn or soy oil. It is necessary to complement this study in vitro with the digestibility analysis in vivo, in order to determine the inclusion percentages in feeds and costs and therefore to develop an efficient feed formulation for the aquaculture of A. trimaculatus.

中文翻译：

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三斑慈鲷 Amphilophus trimaculatus 饲料成分中蛋白质和脂质的潜在生物利用度：体外评估

对于三斑慈鲷 (Amphilophus trimaculatus) 的商业养殖，重要的是要有一套适用于饲料生产的蛋白质和脂质成分，从而以可承受的成本优化养殖。体外消化率通过 pH-stat 技术评估，使用来自 A.trimaculatus 幼鱼的胃和肠多酶提取物。蛋白质成分的消化在酸性和碱性条件下进行，在水解结束时量化游离氨基酸的总量，而脂质成分的消化仅在碱性条件下进行。该品种的消化酶对牛肉粉、鱼粉、优质鸡粉等动物源性原料具有较好的亲和力，具有较好的消化率。可以使用的最佳脂质成分是鱼油。但是，可以使用植物来源的成分，例如大豆或谷物，以及植物油，例如橄榄油、玉米或豆油。有必要通过体内消化率分析来补充这项体外研究，以确定饲料中的包含百分比和成本，从而为三斑曲霉菌的水产养殖开发有效的饲料配方。