In this work, a mixed strain which could degrade hydrolyzed polyacrylamide (HPAM) was constructed based on SS5 and SS6 isolated from two wastewater samples. SS5 is a globular gram‐negative bacterium, and SS6 is a rod‐shaped gram‐negative bacterium. Moreover, SS5 and SS6 were identified as Acinetobacter sp. and Pseudomonas sp., respectively. The removal performance of the mixed strain was optimized using single‐factor experiment and response surface methodology. The maximal HPAM removal efficiency 29.78% was observed at pH 7.5, 35°C, 170 rpm rotation speed, and inoculum loading 3% (v/v) for 7 days. The optimal concentrations of nutrients were 0.32 g/L glucose, 0.21 g/L NaNO₃, 2.10 g/L KH₂PO₄‐K₂HPO₄, and the HPAM removal efficiency reached 45.82%. Finally, the optimal HPAM concentration and the HPAM removal efficiency were 300 mg/L and 45.82%, respectively. The results indicate that the mixed strain was a potential candidate for dealing with oil‐ or gas‐field sewage with high hydrolyzed polyacrylamide concentration.

中文翻译：

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从废水中分离出的混合细菌处理部分水解的聚丙烯酰胺

在这项工作中，基于从两个废水样品中分离出的SS5和SS6，构建了可降解水解聚丙烯酰胺（HPAM）的混合菌株。SS5是球状革兰氏阴性细菌，SS6是棒状革兰氏阴性细菌。此外，SS5和SS6被鉴定为不动杆菌属。和假单胞菌属。使用单因素实验和响应面方法优化了混合菌株的去除性能。在pH 7.5、35°C，170 rpm的转速和3％（v / v）的接种量下连续7天观察到最大的HPAM去除效率29.78％。营养物的最佳浓度分别为0.32克/升葡萄糖，0.21克/升的NaNO ₃，2.10克/升KH ₂ PO ₄ -K ₂HPO ₄，HPAM去除效率达到45.82％。最后，最佳HPAM浓度和HPAM去除效率分别为300 mg / L和45.82％。结果表明，混合菌株是处理高水解聚丙烯酰胺浓度的油田或天然气田污水的潜在候选者。