The aim of this study was to investigate the effects of the incorporation of elastin‐like polypeptide (ELP) on the adhesion maturation of mineral trioxide aggregates (MTA). Two types of ELPs (V125 and V125E8) were genetically synthesized. V125 consisted of 125 repeating pentapeptides (Val‐Pro‐Gly‐Xaa‐Gly) and V125E8 was functionalized with octaglutamic acid in the C‐terminus of V125; both were diluted to 10 wt% in solution. Three 1.5 mm diameter holes in dentin discs were filled with MTA mixed with either a solution of ELP or deionized water. Push‐out bond strength tests were performed following storage in simulated body fluid (SBF) for 1, 2, 4, and 8 weeks (n = 12). The interface between dentin and MTA was observed via scanning electron microscopy (SEM). The maturation of MTA was evaluated with a stereoscopic microscope and SEM. The incorporation of a specific ELP (V125E8) significantly increased the bond strength of MTA to dentin with regard to every maturation period (p < 0.05). The bond strength of MTA also significantly increased with a longer maturation time irrespective of ELP incorporation (p < 0.05). V125E8‐incorporated MTA exhibited a more intimate interface with dentin compared to the other groups. More spindle‐shaped crystal structures and thicker crystals were observed in all MTA mixtures as the storage duration increased even though V125E8 exhibited fewer crystal structures on the surface. Within the limitations of this study, the incorporation of V125E8 increased the adhesive properties of MTA and the maturation of MTA occurred regardless of ELP incorporation.

中文翻译：

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弹性蛋白样多肽掺入对矿物三氧化物聚集体粘附成熟的影响。

本研究的目的是研究弹性蛋白样多肽 (ELP) 的掺入对矿物三氧化物聚集体 (MTA) 粘附成熟的影响。基因合成了两种类型的 ELP（V125 和 V125E8）。V125 由 125 个重复的五肽（Val-Pro-Gly-Xaa-Gly）组成，V125E8 在 V125 的 C 端被八谷氨酸功能化；两者均在溶液中稀释至 10 wt%。牙本质盘中的三个直径为 1.5 毫米的孔填充了与 ELP 溶液或去离子水混合的 MTA。在模拟体液 (SBF) 中储存 1、2、4 和 8 周后进行推出粘合强度测试 ( n= 12）。通过扫描电子显微镜 (SEM) 观察牙本质和 MTA 之间的界面。MTA 的成熟度通过立体显微镜和 SEM 进行评估。就每个成熟期而言，特定 ELP (V125E8) 的掺入显着增加了 MTA 与牙本质的粘合强度 ( p  < 0.05)。无论 ELP 掺入如何，MTA 的粘合强度也随着成熟时间的延长而显着增加（p < 0.05)。与其他组相比，包含 V125E8 的 MTA 与牙本质的界面更加紧密。随着储存时间的增加，在所有 MTA 混合物中观察到更多的纺锤形晶体结构和更厚的晶体，尽管 V125E8 在表面上表现出更少的晶体结构。在本研究的局限性内，V125E8 的掺入增加了 MTA 的粘合性能，并且 MTA 的成熟发生与 ELP 掺入无关。