The gelatin film has great potential in biomedical applications, especially in wound healing. The combination of gelatin films and stem cells could further accelerate the skin regeneration. Although polysaccharide modification can improve the mechanical property and biological activity of gelatin films, information about the stability and cellular affinity is still limited. This study investigated the influence of polysaccharides on the stability and cellular affinity of gelatin films. Two kinds of gelatin‐polysaccharide composite films, including gelatin‐hyaluronic acid (G‐HA) and gelatin‐chitosan (G‐CS), were prepared in this study. It was found that G‐HA composite film had better short‐term and long‐term stability compared with G‐CS composite film. And G‐HA composite film also had better biological safety than G‐CS film. Moreover, the surface of G‐HA composite film supported the adhesion and growth of human umbilical cord Wharton's jelly‐derived mesenchymal stem cells (WJ MSCs) better than G‐CS film surface. These data illustrated that G‐HA composite film has better stability and cellular affinity compared with G‐CS film, which could be considered a promising delivery system of stem cells for further in vivo studies. Therefore, this work would be very helpful to optimize the preparation of gelatin‐polysaccharide composite films.

中文翻译：

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明胶-多糖复合膜的稳定性和细胞亲和性研究[J].

明胶薄膜在生物医学应用中具有巨大的潜力，尤其是在伤口愈合方面。明胶膜和干细胞的结合可以进一步加速皮肤再生。虽然多糖修饰可以提高明胶薄膜的机械性能和生物活性，但关于稳定性和细胞亲和力的信息仍然有限。本研究调查了多糖对明胶薄膜稳定性和细胞亲和力的影响。本研究制备了明胶-透明质酸（G-HA）和明胶-壳聚糖（G-CS）两种明胶-多糖复合膜。结果表明，与G-CS复合膜相比，G-HA复合膜具有更好的短期和长期稳定性。并且G-HA复合膜也比G-CS膜具有更好的生物安全性。而且，G-HA复合膜的表面比G-CS膜表面更好地支持人脐带Wharton's jelly-derived mesenchymal stem cells (WJ MSCs)的粘附和生长。这些数据表明，与 G-CS 膜相比，G-HA 复合膜具有更好的稳定性和细胞亲和力，可被认为是进一步体内研究的有前途的干细胞递送系统。因此，这项工作将有助于优化明胶-多糖复合薄膜的制备。这可以被认为是用于进一步体内研究的有前途的干细胞递送系统。因此，这项工作将有助于优化明胶-多糖复合薄膜的制备。这可以被认为是用于进一步体内研究的有前途的干细胞递送系统。因此，这项工作将有助于优化明胶-多糖复合薄膜的制备。