Chemists and industrialists are continuously attempting to develop greener and more environmentally benign chemical processes to extract essential oils and bioactive metabolites of high purity, finding various applications in cosmetics, detergents, nutraceuticals and pharmaceuticals. An increase preferenced for natural products over synthetic ones has made supercritical fluid technology a primary alternative for the generation of high‐value bioactive ingredients. This effective technique requires only moderate temperatures, eliminates clean‐up steps and avoids the use of harmful organic solvents. In this context, our study was focused on the chemical analysis of Calamintha nepeta subsp. nepeta aromatic extracts obtained with supercritical carbon dioxide. The effect of different operating conditions on the capacity of the lipophilic solvent to extract the targeted volatile components was also studied. The process was carried out at a fairly low constant temperature of 40°C, and with varying the pressure from 90 to 300 bar. The chemical composition of the extracts was analyzed by gas chromatography–mass spectroscopy. The results showed that the composition pattern, the concentrations of individual components and the quality of the extractable analytes were affected by pressure increase. The extraction yields varied from 0.73 to 1.21 wt% at 90 and 300 bar, respectively.

中文翻译：

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压力对Calamintha nepeta亚种超临界二氧化碳提取物特性的影响。荆芥。

化学家和工业家正在不断尝试开发更绿色，对环境更有益的化学工艺，以提取高纯度的精油和生物活性代谢物，从而在化妆品，洗涤剂，营养食品和药品中找到了各种应用。与合成产品相比，对天然产品的偏爱有所增加，已使超临界流体技术成为生成高价值生物活性成分的主要选择。这项有效的技术仅需适中的温度，无需清理步骤，并且避免使用有害的有机溶剂。在这种情况下，我们的研究集中在荆芥子叶亚种的化学分析上。荆芥用超临界二氧化碳获得的芳香提取物。还研究了不同操作条件对亲脂性溶剂提取目标挥发性成分的能力的影响。该过程在相当低的40°C恒定温度下进行，压力从90 bar变为300 bar。提取物的化学成分通过气相色谱-质谱分析。结果表明，压力升高会影响组成模式，各个组分的浓度和可萃取分析物的质量。在90巴和300巴下，萃取产率分别为0.73至1.21重量％。