Abstract Processive endoglucanase EG1 and its core domain, EG1(CD), were used to pretreat the commercial dissolving pulp to improve cellulose reactivity. The Fock reactivity of the pulp which was treated with EG1 and EG1 (CD) at 50 U/g enzyme loading increased from 74.3% of the control to 90.6% and 88.4%, respectively. Refining also improved the Fock reactivity of the pulp, but not as effective as EG1 or EG1(CD) treatment. Refining prior to EG1 or EG1(CD) treatment could slightly further improve the Fock reactivity, to 91.6% and 90.0%, respectively. After enzymatic treatment and (or) refining, the water retention value, differential scanning calorimetry and alkaline solubility analysis indicated that enzyme treatment, especially by EG1, significantly increased the accessibility of fibers to reaction reagents. Combined with the characteristics of soluble reducing sugar produced by EG1 treatment and the changes of degree of polymerization, it is inferred that a small fraction of cellulose crystallization regions are destroyed in the enzymatic hydrolysis process due to the processive acting ability of EG1, and some microchannels in the fiber cell wall were created, which is similar to the effect of “drilling holes”, so that the reaction reagent can reach the inside of the cell wall evenly, thus obviously improving the reactivity of the dissolved pulp.

中文翻译：

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草履虫的持续性内切葡聚糖酶 EG1 酶促预处理改变了超分子结构并提高了溶解浆的反应性

摘要 使用持续性内切葡聚糖酶 EG1 及其核心结构域 EG1(CD) 预处理商业溶解浆以提高纤维素反应性。用 EG1 和 EG1 (CD) 以 50 U/g 酶负载量处理的纸浆的 Fock 反应性分别从对照的 74.3% 增加到 90.6% 和 88.4%。精炼也提高了纸浆的 Fock 反应性，但不如 EG1 或 EG1(CD) 处理有效。在 EG1 或 EG1(CD) 处理之前进行精炼可以稍微进一步提高 Fock 反应性，分别达到 91.6% 和 90.0%。酶处理和（或）精制后，保水值、差示扫描量热法和碱溶度分析表明，酶处理，尤其是 EG1，显着增加了纤维对反应试剂的可及性。