In bone cancer treatment, local delivery of chemotherapeutic agents is preferred compared to other routes of administration. Delivery of multiple drugs using biodegradable carriers improves the treatment efficiency and overcomes drug resistance and toxicity. With this approach, we have developed multilayer biodegradable core shell nanoparticles (NPs) using the electro-spraying technique to deliver methotrexate (MTX) and doxorubicin (DOX) for the treatment of osteosarcoma. These core-shell NPs with a mean particle size of 212 ± 41 nm consist of hydroxyapatite (HA) and DOX as core with the outer shell made of chitosan (CH) followed by polycaprolactone (PCL) with MTX. The encapsulation efficiency of MTX was around 85% and DOX was 38%. In vitro drug release studies were performed in phosphate buffered saline (PBS) at pH 5 and pH 7.4 for 8 days. Different release profiles were observed in both acidic and alkaline pH. The sequential release of MTX followed by DOX was observed in both pH in sustained manner. Human osteosarcoma MG 63 (OMG-63) cells lines were used to test the cytotoxicity of drug loaded NPs. Multi-drug encapsulated bioresorbable and biodegradable electro-sprayed core shell NPs will be promising as a bone substitute for the treatment of osteosarcoma.

中文翻译：

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使用电喷雾芯壳纳米载体联合提供抗癌药物，用于骨肉瘤治疗。

在骨癌治疗中，与其他给药途径相比，优选局部给药化学治疗剂。使用可生物降解的载体递送多种药物可提高治疗效率并克服耐药性和毒性。通过这种方法，我们使用电喷雾技术开发了可生物降解的多层核壳纳米颗粒（NPs），以递送甲氨蝶呤（MTX）和阿霉素（DOX）来治疗骨肉瘤。这些平均粒径为212±41 nm的核壳NP由羟基磷灰石（HA）和DOX组成，其外壳由壳聚糖（CH）制成，然后是聚己内酯（PCL）和MTX。MTX的封装效率约为85％，而DOX为38％。在pH 5和pH 7.4的磷酸盐缓冲盐水（PBS）中进行了8天的体外药物释放研究。在酸性和碱性pH下均观察到不同的释放曲线。在两个pH值下均持续观察到MTX和DOX的顺序释放。人类骨肉瘤MG 63（OMG-63）细胞系用于测试载药NPs的细胞毒性。多药封装的可生物吸收和可生物降解的电喷雾核壳纳米粒将有望作为治疗骨肉瘤的骨替代品。