The present study was designed to examine the cytotoxic activity of synthesized single metal oxide nanoparticles such as copper oxide (CuO), iron oxide (γFe₂O₃) and multi-metal oxide zinc, iron and copper oxide (CuZnFe₂O₃) in human breast cancer (MCF-7) cells. These single and multi-metal oxide nanoparticles were characterized by X-ray diffraction (XRD) and scanning electron microscopy (SEM). The cytotoxic potential of these nanoparticles was assessed by MTT and NRU assays. Further, the morphological alterations, reactive oxygen species (ROS) generation and mitochondrial membrane potential (MMP) were also studied. The novelty of the present work express that the MTT and NRU assays revealed a concentration dependent decrease in the viability of MCF-7 cells. The percent cell viability was recorded as 82%, 81%, and 79% in CuFeZn NPs, 81%, 80% and 70% in γFe₂O₃NPs, 54%, 43% and 27% in CuONPs exposed MCF-7 cells for 24 h at doses 25, 50, 100 μg/ml, respectively by MTT assay. The MTT results was also justified by NRU assay. An increase in ROS generation was observed as 21% and 35% in CuOFeZnNPs, 41% and 61% in γFe₂O₃NPs and 54% and 89% in CuONPs and the decrease in MMP level was observed as 14% and 24% in CuOFeZn NPs, 37% and 46% in γFe₂O₃NPs and 52% and 58% in CuONPs at 25 and 50 µg/ml, respectively related to control. Together, these results suggest that the loss of MMP and increase in ROS level could be important mechanism of single and multi-metal oxide nanoparticles induced cytotoxicity in human breast cancer cells.

本研究的目的是检查合成的单金属氧化物纳米颗粒，如氧化铜（CuO），氧化铁的细胞毒性活性（γFe ₂ ö ₃）和多金属氧化锌，氧化铁和氧化铜（CuZnFe ₂ ö ₃在人类乳腺癌（MCF-7）细胞中的表达。这些单金属和多金属氧化物纳米颗粒通过X射线衍射（XRD）和扫描电子显微镜（SEM）进行表征。通过MTT和NRU分析评估了这些纳米颗粒的细胞毒性潜力。此外，还研究了形态变化，活性氧（ROS）生成和线粒体膜电位（MMP）。本工作的新颖性表明，MTT和NRU分析揭示了MCF-7细胞活力的浓度依赖性降低。百分比细胞活力在γFe记录为82％，81％，和在CuFeZn的NP 79％，81％，80％和70％的₂ ö ₃通过MTT分析，分别以25、50、100μg/ ml的剂量将CuONP中的NP分别以25、50、100μg/ ml的剂量暴露于MCF-7细胞24小时。NRU分析也证明了MTT结果的合理性。观察到21％和在CuOFeZnNPs 35％，41％和在γFe61％在ROS产生的增加_2个ö ₃纳米颗粒和54％，而在CuONPs 89％和在MMP水平的降低，观察到14％和24％ CuOFeZn NPs在25和50 µg / ml时分别在γFe2 O ₃ NPs中占37％和46％，在CuONPs中_分别占52％和58％。总之，这些结果表明，MMP的丢失和ROS水平的升高可能是单金属氧化物和多金属氧化物纳米粒子在人乳腺癌细胞中诱导细胞毒性的重要机制。