Ligands bound to protein assemblies provide critical information for function, yet are often difficult to capture and define. Here we develop a top-down method, 'nativeomics', unifying 'omics' (lipidomics, proteomics, metabolomics) analysis with native mass spectrometry to identify ligands bound to membrane protein assemblies. By maintaining the link between proteins and ligands, we define the lipidome/metabolome in contact with membrane porins and a mitochondrial translocator to discover potential regulators of protein function.

中文翻译：

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结合天然质谱和“组学”质谱来鉴定与膜蛋白结合的内源配体。


与蛋白质组装体结合的配体提供了功能的关键信息，但通常难以捕获和定义。在这里，我们开发了一种自上而下的方法，即“天然组学”，将“组学”（脂质组学、蛋白质组学、代谢组学）分析与天然质谱分析相结合，以识别与膜蛋白组装体结合的配体。通过维持蛋白质和配体之间的联系，我们定义了与膜孔蛋白和线粒体易位蛋白接触的脂质组/代谢组，以发现蛋白质功能的潜在调节因子。