Research question

What is the expression pattern of microRNA (miRNA) in exosomes isolated from eutopic endometrial stromal cells (EuESC) of women with endometriosis-associated infertility?

Design

Small RNA sequencing was conducted in exosomes isolated from EuESC of women with endometriosis-associated infertility (n = 3) and normal endometrial stromal cells (NESC) of fertile women without endometriosis (n = 3). The differentially expressed miRNA in exosomes derived from EuESC and NESC were identified. The functions of the differentially expressed miRNA were analysed by gene ontology enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis.

Results

Small RNA sequencing showed that the percentages of exosomal miRNA in the total small RNA isolated from EuESC and NESC were not significantly different (P = 0.7804). A total of 49 differentially expressed miRNA (fold change >1.5 and P < 0.05) were identified, including 26 up-regulated and 23 down-regulated in EuESC exosomes as compared with NESC exosomes. Functional analysis revealed that 12 miRNA were predicted to target homeobox A10 (HOXA10) and/or the leukaemia inhibitory factor (LIF) 3’ untranslated region (UTR). Both HOXA10 and LIF mRNA expression levels were significantly decreased in EuESC compared with NESC (P = 0.0222 and 0.0395, respectively). In addition, the predicated target genes of these differentially expressed exosomal miRNA were significantly (P < 0.05) enriched in 76 pathways, including the MAPK and Wnt signalling pathways.

Conclusions

The differential expression patterns of exosomal miRNA were identified. Many exosomal miRNA may be involved in regulating the endometrial receptivity of women with endometriosis-associated infertility.

中文翻译：

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microRNA在子宫内膜异位症相关不育妇女子宫内膜基质细胞外泌体中的差异表达。

研究问题

从患有子宫内膜异位症相关性不育的女性的异位子宫内膜基质细胞（EuESC）中分离出的外泌体中microRNA（miRNA）的表达模式是什么？

设计

小RNA测序从妇女的EuESC分离子宫内膜异位症相关的不育症（外来体进行Ñ 育龄妇女= 3）和正常子宫内膜间质细胞（NESC）子宫内膜异位症无（Ñ  = 3）。鉴定了在源自EuESC和NESC的外来体中差异表达的miRNA。通过基因本体富集分析和《京都基因与基因组百科全书》（KEGG）途径分析来分析差异表达的miRNA的功能。

结果

小RNA测序表明，从EuESC和NESC分离出的小RNA总量中外泌体miRNA的百分比无显着差异（P  = 0.7804）。 鉴定出总共49个差异表达的miRNA（倍数变化> 1.5和P <0.05），其中与NESC外泌体相比，EuESC外泌体中有26个上调而23个下调。功能分析表明，预计有12个miRNA靶向同源盒A10（HOXA10）和/或白血病抑制因子（LIF）3'非翻译区（UTR）。既HOXA10和LIF mRNA的表达水平在EuESC均显著降低与NESC相比（P 分别为0.0222和0.0395）。此外，这些差异表达的外泌体miRNA的预测目标基因显着（P  <0.05）富集了76条通路，包括MAPK和Wnt信号通路。

结论

鉴定了外泌体miRNA的差异表达模式。许多外泌体miRNA可能参与调节子宫内膜异位症相关不育妇女的子宫内膜容受性。