Ectoine production using inexpensive and renewable biomass resources has attracted great interest among the researchers due to the low yields of ectoine in current fermentation approaches that complicate the large-scale production of ectoine. In this study, ectoine was produced from corn steep liquor (CSL) and soybean hydrolysate (SH) in replacement to yeast extract as the nitrogen sources for the fermentation process. To enhance the bacterial growth and ectoine production, biotin was added to the Halomonas salina fermentation media. In addition, the effects addition of surfactants such as Tween 80 and saponin on the ectoine production were also investigated. Results showed that both the CSL and SH can be used as the nitrogen source substitutes in the fermentation media. Higher amount of ectoine (1781.9 mg L⁻¹) was produced in shake flask culture with SH-containing media as compared to CSL-containing media. A total of 2537.0 mg L⁻¹ of ectoine was produced at pH 7 when SH-containing media was applied in the 2 L batch fermentation. Moreover, highest amount of ectoine (1802.0 mg L⁻¹) was recorded in the SH-containing shake flask culture with addition of 0.2 μm mL⁻¹ biotin. This study demonstrated the efficacy of industrial waste as the nutrient supplement for the fermentation of ectoine production.

使用廉价且可再生的生物质资源生产四氢嘧啶引起了研究人员的极大兴趣，因为当前发酵方法中四氢嘧啶的产量较低，这使得四氢嘧啶的大规模生产变得复杂。在本研究中，用玉米浆（CSL）和大豆水解物（SH）生产四氢嘧啶，以替代酵母提取物作为发酵过程的氮源。为了增强细菌生长和四氢嘧啶产量，将生物素添加到盐单胞菌发酵培养基中。此外，还考察了吐温80、皂苷等表面活性剂的添加对四氢嘧啶生产的影响。结果表明，CSL和SH均可作为发酵培养基中的氮源替代品。与含有CSL的培养基相比，在含有SH的培养基的摇瓶培养中产生了更高量的四氢嘧啶（1781.9 mg L ^-1 ）。当在2L分批发酵中应用含有SH的培养基时，在pH 7时总共产生了2537.0mg L ^-1的四氢嘧啶。此外，在添加0.2 μm mL ^-1生物素的含有SH的摇瓶培养物中记录到最高量的四氢嘧啶（1802.0 mg L ^-1 ）。这项研究证明了工业废物作为四氢嘧啶发酵生产的营养补充剂的功效。