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A new insight into the intestine of Pacific white shrimp: Regulation of intestinal homeostasis and regeneration in Litopenaeus vannamei during temperature fluctuation.
Comparative Biochemistry and Physiology D: Genomics & Proteomics ( IF 2.2 ) Pub Date : 2020-05-03 , DOI: 10.1016/j.cbd.2020.100687 Zhenlu Wang 1 , Jiang Zhou 1 , Junyi Li 1 , Wei Lv 2 , Jixing Zou 1 , Lanfen Fan 1
Comparative Biochemistry and Physiology D: Genomics & Proteomics ( IF 2.2 ) Pub Date : 2020-05-03 , DOI: 10.1016/j.cbd.2020.100687 Zhenlu Wang 1 , Jiang Zhou 1 , Junyi Li 1 , Wei Lv 2 , Jixing Zou 1 , Lanfen Fan 1
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Litopenaeus vannamei (L. vannamei) is an essential aquaculture shrimp throughout the world, but its aquaculture industry is threatened by temperature fluctuation. In this study, our histological results indicated that the shrimp intestine has a self-repairing ability during temperature fluctuation; however the potential mechanisms were still unknown. Therefore, transcriptome profiles of the intestine were collected from shrimp at 28 °C (C28), 13 °C (T13) and 28 °C after their temperature rose back (R28) and were analyzed. A total of 2229 differentially expressed genes (DEGs) (986 up- and 1243 downregulated) were identified in the C28 group, and 1790 DEGs (933 up- and 857 downregulated) were identified in the R28 group when compared to their expression levels in the T13 group. According to the functional annotation using KEGG, we found that the immune system was the most enriched section of organismal systems and that the shrimp can mobilize the body's immune response to regulate organism homeostasis during temperature fluctuation, although cold stress decreased the immunity. Additionally, metabolic inhibition is a strategy to cope with cold stress, and the regulation of lipid metabolism was especially important for shrimp during temperature fluctuation. Remarkably, the Hippo signaling pathway might help the repair of intestinal structure. Our research provides the first histological analysis and transcriptome profiling for the L. vannamei intestine during the temperature fluctuation stage. These results enrich our understanding of the mechanism of intestinal self-repair and homeostasis and could provide guidance for shrimp farming.
中文翻译：
对太平洋白虾肠道的新见解：温度波动期间南美白对虾肠道稳态和再生的调节。
南美白对虾（L. vannamei）是全世界重要的水产养殖虾，但其水产养殖业受到温度波动的威胁。在本研究中，我们的组织学结果表明，虾肠在温度波动时具有自我修复的能力。然而，潜在的机制仍然未知。因此，在虾的温度回升（R28）后，分别从虾在28°C（C28），13°C（T13）和28°C的温度下收集虾的转录组谱，并进行分析。与在C28组中的表达水平相比，在C28组中总共鉴定出2229个差异表达基因（DEGs）（986个上调和1243个下调），在R28组中鉴定出1790个DEGs（933个上调和857个下调）。 T13组。根据使用KEGG的功能注释，我们发现免疫系统是机体系统中最丰富的部分，尽管冷应激会降低免疫力，但虾可以在温度波动时调动人体的免疫反应来调节机体稳态。此外，代谢抑制是应对冷胁迫的一种策略，脂质代谢的调节对于温度波动期间虾尤其重要。值得注意的是，河马信号通路可能有助于肠道结构的修复。我们的研究提供了在温度波动阶段南美白对虾肠的首次组织学分析和转录组分析。这些结果丰富了我们对肠道自我修复和体内平衡机制的理解，并可以为虾类养殖提供指导。
更新日期：2020-05-03
中文翻译：
对太平洋白虾肠道的新见解：温度波动期间南美白对虾肠道稳态和再生的调节。
南美白对虾（L. vannamei）是全世界重要的水产养殖虾，但其水产养殖业受到温度波动的威胁。在本研究中，我们的组织学结果表明，虾肠在温度波动时具有自我修复的能力。然而，潜在的机制仍然未知。因此，在虾的温度回升（R28）后，分别从虾在28°C（C28），13°C（T13）和28°C的温度下收集虾的转录组谱，并进行分析。与在C28组中的表达水平相比，在C28组中总共鉴定出2229个差异表达基因（DEGs）（986个上调和1243个下调），在R28组中鉴定出1790个DEGs（933个上调和857个下调）。 T13组。根据使用KEGG的功能注释，我们发现免疫系统是机体系统中最丰富的部分，尽管冷应激会降低免疫力，但虾可以在温度波动时调动人体的免疫反应来调节机体稳态。此外，代谢抑制是应对冷胁迫的一种策略，脂质代谢的调节对于温度波动期间虾尤其重要。值得注意的是，河马信号通路可能有助于肠道结构的修复。我们的研究提供了在温度波动阶段南美白对虾肠的首次组织学分析和转录组分析。这些结果丰富了我们对肠道自我修复和体内平衡机制的理解，并可以为虾类养殖提供指导。
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