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Establishment of a Japanese medaka (Oryzias latipes) transgenic line expressing Takifugu rubripes pufferfish saxitoxin and tetrodotoxin binding protein 1, and evaluation of tributyltin toxicity via in ovo nanoinjection.
Comparative Biochemistry and Physiology C: Toxicology & Pharmacology ( IF 3.9 ) Pub Date : 2020-05-03 , DOI: 10.1016/j.cbpc.2020.108785 Yuki Takai 1 , Naohiro Mizoguchi 1 , Masato Kinoshita 2 , Xuchun Qiu 3 , Yohei Shimasaki 1 , Yuji Oshima 4
Comparative Biochemistry and Physiology C: Toxicology & Pharmacology ( IF 3.9 ) Pub Date : 2020-05-03 , DOI: 10.1016/j.cbpc.2020.108785 Yuki Takai 1 , Naohiro Mizoguchi 1 , Masato Kinoshita 2 , Xuchun Qiu 3 , Yohei Shimasaki 1 , Yuji Oshima 4
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Pufferfish saxitoxin and tetrodotoxin binding proteins (PSTBPs) play an important role in the toxification of certain species of pufferfish. Recombinant Takifugu rubripes PSTBP1 (rTrub.PSTBP1) is reported to bind to tributyltin, and so it has been suggested that rTrub.PSTBP1 may reduce the toxicity of tributyltin. However, the role of PSTBP1 in vivo remains to be elucidated. Here, we established a transgenic medaka line showing whole-body Renilla reniformis green fluorescent protein and Trub.PSTBP1 expression, as confirmed by real-time polymerase chain reaction and mRNA-Seq analysis. mRNA-Seq analysis also showed that cytochrome P450 superfamily genes and the gene encoding ATP-binding cassette sub-family G member 2 were highly expressed in the transgenic medaka. Using embryos of the transgenic medaka line, we conducted an in ovo nanoinjection test to examine the effect of Trub.PSTBP1 in vivo, and obtained data suggesting that Trub.PSTBP1 expression may have reduced the toxicity of tributyltin in our transgenic medaka line. Our findings will be useful for future functional analyses of Trub.PSTBP1.
中文翻译：
表达线虫河豚毒素和河豚毒素结合蛋白1的日本（Oryzias latipes）转基因品系的建立，并通过卵内纳米注射评价三丁基锡的毒性。
河豚毒素和河豚毒素结合蛋白（PSTBP）在某些河豚毒素的毒化中起重要作用。据报道重组塔基革红宝石PSTBP1（rTrub.PSTBP1）与三丁基锡结合，因此有人提出rTrub.PSTBP1可以降低三丁基锡的毒性。但是，PSTBP1在体内的作用仍有待阐明。在这里，我们建立了一个转基因花aka品系，通过实时聚合酶链反应和mRNA-Seq分析证实了该基因，显示了整个肾形肾病绿色荧光蛋白和Trub.PSTBP1表达。mRNA-Seq分析还表明，细胞色素P450超家族基因和编码ATP结合盒亚家族G成员2的基因在转基因中高表达。使用转基因高种系的胚胎，我们进行了卵内纳米注射测试，以观察Trub.PSTBP1在体内的作用，并获得数据表明Trub.PSTBP1的表达可能降低了我们转基因genic高品系中三丁基锡的毒性。我们的发现将对Trub.PSTBP1的未来功能分析有用。
更新日期：2020-05-03
中文翻译：
表达线虫河豚毒素和河豚毒素结合蛋白1的日本（Oryzias latipes）转基因品系的建立，并通过卵内纳米注射评价三丁基锡的毒性。
河豚毒素和河豚毒素结合蛋白（PSTBP）在某些河豚毒素的毒化中起重要作用。据报道重组塔基革红宝石PSTBP1（rTrub.PSTBP1）与三丁基锡结合，因此有人提出rTrub.PSTBP1可以降低三丁基锡的毒性。但是，PSTBP1在体内的作用仍有待阐明。在这里，我们建立了一个转基因花aka品系，通过实时聚合酶链反应和mRNA-Seq分析证实了该基因，显示了整个肾形肾病绿色荧光蛋白和Trub.PSTBP1表达。mRNA-Seq分析还表明，细胞色素P450超家族基因和编码ATP结合盒亚家族G成员2的基因在转基因中高表达。使用转基因高种系的胚胎，我们进行了卵内纳米注射测试，以观察Trub.PSTBP1在体内的作用，并获得数据表明Trub.PSTBP1的表达可能降低了我们转基因genic高品系中三丁基锡的毒性。我们的发现将对Trub.PSTBP1的未来功能分析有用。
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