In this study, an aqueous two-phase micellar system (ATPMS), formed by the non-ionic surfactant Triton X-114, was used to investigate the partitioning of cellulolytic enzymes produced by the filamentous fungus Aspergillus fumigatus CCT 7873. Performance of the ATPMS on the partitioning of CMCase (activity on carboxymethyl cellulose) and FPase (activity on filter paper) was investigated by varying the temperature (35, 40, 45, 50, 55, 60, and 65 °C), enzyme crude extract concentration (20, 40, 60, and 80% w/w), and Triton X-114 concentration (2, 4, 6, and 8% w/w) and by adding different inorganic salts (NaCl, CaCl2, MgSO4, and MnSO4) in the system. An ATPMS formed with 8% (w/w) Triton X-114 and 40% (w/w) enzymatic crude extract at a system temperature of 55 °C was most favorable for partitioning the tested enzymes. Under these conditions, a purification factor for CMCase and FPase of 10.89 and 0.65 was reached, respectively. The addition of inorganic salts changed the distribution of enzymes. Of these, CaCl2 contributed to a higher distribution coefficient (50.0), whereas for FPase, the presence of MnSO4 in the system improved the purification factor to 3.94. The highest values obtained for the yield and purification factors demonstrate that ATPMS is an interesting option for recovering and purifying cellulolytic enzymes.

中文翻译：

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使用水相两相胶束系统从烟曲霉CCT 7873中回收和纯化纤维素分解酶

在这项研究中，由非离子表面活性剂Triton X-114形成的水相两相胶束系统（ATPMS）用于研究由丝状真菌烟曲霉CCT 7873产生的纤维素分解酶的分配。ATPMS的性能通过改变温度（35、40、45、50、55、60和65°C），酶粗提液浓度（20）来研究CMCase（对羧甲基纤维素的活性）和FPase（对滤纸的活性）的分配，40、60和80％w / w），Triton X-114浓度（2、4、6和8％w / w），并在其中添加不同的无机盐（NaCl，CaCl2，MgSO4和MnSO4）系统。在系统温度为55°C时，由8％（w / w）Triton X-114和40％（w / w）酶促粗提物形成的ATPMS最适合分配测试的酶。在这种情况下 CMCase和FPase的纯化因子分别达到10.89和0.65。无机盐的添加改变了酶的分布。其中，CaCl2有助于提高分配系数（50.0），而对于FPase，系统中MnSO4的存在将纯化系数提高至3.94。获得的产率和纯化因子的最高值表明，ATPMS是回收和纯化纤维素分解酶的有趣选择。