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Amino acids and fatty acids composition in mass‐cultured Teleaulax amphioxeia strains with notable potential for rotifer (Brachionus plicatilis) enrichment
Journal of the World Aquaculture Society ( IF 2.3 ) Pub Date : 2020-05-03 , DOI: 10.1111/jwas.12698
Hyung Seop Kim 1 , Jong Hyeok Kim 2 , Soo‐Gun Jo 1 , Jung‐Rae Rho 1 , Wonho Yih 1
Affiliation  

To evaluate the nutritional quality of the Teleaulax/Plagioselmis/Geminigera (TPG) clade species over the conventional prey species of the Rhodomonas/Rhinomonas/Storeatula (RHO) clade for the enrichment of a rotifer, Brachionus plicatilis, two strains of Teleaulax amphioxeia (TA), CR‐MAL07 and CR‐MAL08‐02, and a Chroomonas sp. strain CR‐MAL10 from Korean coastal waters were mass cultured in 500 L photobioreactors. When harvested from the stationary growth phase, the composition of all three macronutrients and three n‐3 highly unsaturated fatty acids (UFAs) exhibited large deviations among strains and culture media. The composition of essential amino acids and UFAs was higher in CR‐08‐02 by up to 1.1 and 22.2 times, respectively, than that in a commercially available Chlorella sp. strain. For the enrichment of B. plicatilis cultures previously grown with the Chlorella concentrates, high‐density cultures of CR‐08‐02 were offered. After a 3‐hr enrichment, the compositions of eicosapentaenoic acid, n‐3 FA (fatty acids), and n‐6 FA in B. plicatilis increased by 331, 19.5, and 10.48%, respectively, which might reflect the relevant quality compositions in the TA cells. Thus, TA in the TPG clade can be another novel type of prey to enrich rotifer cultures for the marine larviculture.

中文翻译:

大量培养的轮虫(Brachionus plicatilis)富集潜力的Teleaulax amphioxeia菌株中的氨基酸和脂肪酸组成

评估Teleaulax / Plagioselmis / Geminigera(TPG)进化枝物种比Rhodomonas / Rhinomonas / Storeatula(RHO)进化枝的常规猎物物种的营养质量,以富集轮虫,Brachionus plicatilis和两株Teleaulax amphioxeiaTA)),CR‐MAL07和CR‐MAL08-02和Chroomonassp。将来自韩国沿海水域的CR‐MAL10菌株在500 L光生物反应器中进行大规模培养。从静止生长期收获时,所有三种常量营养素和三种n-3高不饱和脂肪酸(UFA)的组成在菌株和培养基之间均表现出较大偏差。CR-08-02中必需氨基酸和UFA的组成分别比市售小球藻(Chlorella sp。)高1.1倍和22.2倍。应变。为了丰富以前使用小球藻浓缩物生长的褶皱芽孢杆菌培养物,提供了高密度的CR-08-02培养物。浓缩3小时后,褶皱芽孢杆菌中二十碳五烯酸,n-3 FA(脂肪酸)和n-6 FA的组成分别增加了331、19.5和10.48%,这可能反映了TA细胞中相关的质量组成。因此,TPG进化枝中的TA可能是另一种新颖的猎物,可以丰富轮虫养殖的海洋幼虫。
更新日期:2020-05-03
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