Nitrogen-limiting condition is essential for citric acid production by Yarrowia lipolytica. Mitochondrial protein expression profiles of Y. lipolytica IMUFRJ 50,682 cells cultivated in biomass proliferation medium (YPG medium, yeast extract, peptone and glycerol) and citric acid production medium (CA medium) were analyzed to identify differences in expressed proteins in response to medium composition. The identification of 45 proteins in mitochondria of YPG medium cells and 48 proteins in mitochondria of CA medium cells were possible with proteomic analyses. Only 11 proteins were common to both conditions, showing a different expression pattern in relation to limiting and non-limiting nitrogen conditions. For both conditions, most proteins (52%-CA medium, 46%-YPG medium) were related to energy metabolism. CA medium cells expressed more carbohydrate metabolism proteins (six proteins) then YPG medium cells (three proteins) and the opposite was detected for translation proteins.

中文翻译：

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调查解脂耶氏酵母的线粒体蛋白质表达谱响应柠檬酸生产。

氮限制条件对于解脂耶氏酵母生产柠檬酸至关重要。分析了在生物质增殖培养基（YPG培养基，酵母提取物，蛋白ept和甘油）和柠檬酸生产培养基（CA培养基）中培养的解脂耶氏酵母IMUFRJ 50,682细胞的线粒体蛋白质表达谱，以鉴定表达的蛋白质对培养基组成的差异。通过蛋白质组学分析可以鉴定YPG培养基细胞线粒体中的45种蛋白质和CA培养基细胞线粒体中的48种蛋白质。在这两种条件下只有11种蛋白质是共同的，相对于限制性和非限制性氮条件显示出不同的表达模式。对于这两种情况，大多数蛋白质（52％-CA培养基，46％-YPG培养基）与能量代谢有关。