N⁶-methyladenosine (m⁶A) is the most common type of eukaryotic mRNA modification. It plays an important role in regulating plant growth and development and stress resistance. m⁶A modification influences nearly all aspects of RNA metabolism and functionality and has great potential for improving crop quality. However, changing m⁶A modification levels as a whole may have unpredictable effects, making it impossible to accurately predict the effect of specific m⁶A modifications on RNA. In this opinion article, the main challenges and possible solutions for exploring m⁶A modification functions in plant systems are discussed. An m⁶A editing platform that uses new high-throughput methods to identify m⁶A modification at single-base resolution, and genome editing for selective editing of specific m⁶A sites for crop improvement is proposed.

N ^6-甲基腺苷（m ⁶ A）是真核mRNA修饰的最常见类型。它在调节植物生长发育和抗逆性中起重要作用。m ⁶修饰几乎影响RNA代谢和功能的所有方面，并具有改善作物质量的巨大潜力。但是，总体上改变m ⁶ A修饰水平可能会产生不可预测的影响，从而无法准确预测特定m ⁶ A修饰对RNA的影响。在这篇观点文章中，讨论了探索工厂系统中m ⁶ A修改功能的主要挑战和可能的解决方案。安⁶提出了一种编辑平台，该平台使用新的高通量方法以单碱基分辨率识别m ⁶ A修饰，并进行基因组编辑以选择性编辑特定m ⁶ A位点以改善作物。