Sugarcane is one of the major cash crop whose low productivity is due to low nutrients supply and insect pest attacks. The nutrient requirement may be fulfilled by increasing the amount of fertilizer supplements but pest attack is difficult to control through poisonous chemicals due to their harmful effects on plant growth as well as human health. Recently, sugarcane black bug ( Cavelarius excavates ) was reported to attack sugarcane crop in Pakistan which caused plethora of damages to the crop yield. To control the insect pest, first step is to identify the enemy insect precisely. Therefore, a DNA barcode based study was required for the correct identification of bug species. DNA barcoding is one of the most powerful and sophisticated tools for identifying insects where sequence of mitochondrial gene cytochrome C oxidase I (COI) is used as DNA marker. Sugarcane black bug was sampled and its genomic DNA was subject to PCR for amplification of COI gene. Sanger sequencing of targeted gene was done commercially from Macrogen (South Korea). Sequence analysis revealed that our sequence was first DNA barcode sequence of sugarcane black bug ( Cavelarius excavates ) in the databases. This study will help us to open more ways to understand and controlling sugarcane black bug.

中文翻译：

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使用细胞色素C氧化酶I（COI）基因作为DNA条形码对巴基斯坦甘蔗黑虫（Cavelarius excavates）的分子鉴定

甘蔗是主要的经济作物之一，其生产力低下是由于养分供应不足和虫害侵袭。可以通过增加肥料补充剂的量来满足营养需求，但由于有毒化学品对植物生长和人类健康的有害影响，因此很难通过有毒化学品控制害虫的侵袭。最近，据报道，甘蔗黑虫（Cavelarius excavates）袭击了巴基斯坦的甘蔗作物，对作物产量造成了过多的损害。要控制害虫，首先要准确识别敌虫。因此，需要基于 DNA 条形码的研究才能正确识别昆虫种类。DNA 条形码是识别昆虫的最强大和最复杂的工具之一，其中线粒体基因细胞色素 C 氧化酶 I (COI) 的序列用作 DNA 标记。采集甘蔗黑虫，对其基因组DNA进行PCR扩增COI基因。目标基因的 Sanger 测序是从 Macrogen（韩国）商业完成的。序列分析表明，我们的序列是数据库中第一个甘蔗黑虫（Cavelarius excavates）的DNA条形码序列。这项研究将帮助我们开辟更多了解和控制甘蔗黑虫的方法。序列分析表明，我们的序列是数据库中第一个甘蔗黑虫（Cavelarius excavates）的DNA条形码序列。这项研究将帮助我们开辟更多了解和控制甘蔗黑虫的方法。序列分析表明，我们的序列是数据库中第一个甘蔗黑虫（Cavelarius excavates）的DNA条形码序列。这项研究将帮助我们开辟更多了解和控制甘蔗黑虫的方法。