Approaches for studying the evolution of globular proteins are now well established yet are unsuitable for disordered sequences. Our understanding of the evolution of proteins containing disordered regions therefore lags that of globular proteins, limiting our capacity to estimate their evolutionary history, classify paralogs, and identify potential sequence–function relationships. Here, we overcome these limitations by using new analytical approaches that project representations of sequence space to dissect the evolution of proteins with both ordered and disordered regions, and the correlated changes between these. We use the fasciclin-like arabinogalactan proteins (FLAs) as a model family, since they contain a variable number of globular fasciclin domains as well as several distinct types of disordered regions: proline (Pro)-rich arabinogalactan (AG) regions and longer Pro-depleted regions.

中文翻译：

---

蛋白质家族中序列多样的无序区域的进化：混沌中的秩序。

现在已经很好地研究了研究球状蛋白进化的方法，但不适用于无序序列。因此，我们对包含无序区域蛋白质的进化的理解落后于球状蛋白质的理解，从而限制了我们估计其进化历史，对旁系同源物进行分类以及确定潜在的序列-功能关系的能力。在这里，我们通过使用新的分析方法克服了这些局限性，该分析方法通过投影序列空间的表示来剖析具有有序和无序区域的蛋白质的进化以及它们之间的相关变化。我们将fasciclin样的阿拉伯半乳聚糖蛋白（FLA）用作模型家族，因为它们包含可变数量的球形fasciclin域以及几种不同类型的无序区域：