Rhamnus prinoides (gesho) stem extract prevents co‐culture biofilm formation by Streptococcus mutans and Candida albicans,Letters in Applied Microbiology

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Rhamnus prinoides (gesho) stem extract prevents co‐culture biofilm formation by Streptococcus mutans and Candida albicans
Letters in Applied Microbiology ( IF 2.0 ) Pub Date : 2020-06-18 , DOI: 10.1111/lam.13307
M Campbell ₁ , R Fathi ₁ , S Y Cheng ₁ , A Ho ₁ , E S Gilbert ₁

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Streptococcus mutans and Candida albicans exhibit a symbiotic relationship to form polymicrobial biofilms that exacerbate oral infections including early-childhood caries, periodontitis and candidiasis. Rhamnus prinoides (gesho) has traditionally been used for the treatment of a variety of illnesses and was recently found to inhibit Gram positive bacterial biofilm formation. We hypothesized that Rhamnus prinoides extracts have anti-biofilm activity against S. mutans and C. albicans mono- and dual-species biofilms. Ethanol extracts were prepared from gesho stems and leaves then anti-biofilm activity was assessed using crystal violet, resazurin and XTT staining. Ethanol extracts significantly inhibited Streptococcus mutans and Candida albicans mono-species biofilm formation up to 97% and 75%, respectively. The stem ethanol extract disrupted S. mutans and C. albicans co-culture synergism with 98% less polymicrobial biofilm formation than the untreated control. Additionally, this extract inhibited planktonic S. mutans cell growth and decreased biofilm polysaccharide production up to 99%. The reduction in polysaccharide production is likely a contributing factor in the anti-biofilm activity of GSE. These findings indicate that gesho or gesho-derived compounds may have potential as additives to oral hygiene products.

中文翻译：

Rhamnus prinoides (gesho) 茎提取物防止变形链球菌和白色念珠菌共培养生物膜形成

变形链球菌和白色念珠菌表现出共生关系，形成多种微生物生物膜，加剧口腔感染，包括儿童早期龋齿、牙周炎和念珠菌病。Rhamnus prinoides (gesho) 传统上用于治疗多种疾病，最近发现它可以抑制革兰氏阳性细菌生物膜的形成。我们假设鼠李提取物对 S. mutans 和 C. albicans 单种和双种生物膜具有抗生物膜活性。从 gesho 茎和叶制备乙醇提取物，然后使用结晶紫、刃天青和 XTT 染色评估抗生物膜活性。乙醇提取物显着抑制变形链球菌和白色念珠菌单种生物膜形成，分别高达 97% 和 75%。茎乙醇提取物破坏了 S. 与未处理的对照相比，变形杆菌和白色念珠菌的共培养协同作用减少了 98% 的多微生物生物膜形成。此外，该提取物可抑制浮游变形链球菌细胞生长并减少高达 99% 的生物膜多糖产量。多糖产量的减少可能是 GSE 抗生物膜活性的一个促成因素。这些发现表明 gesho 或 gesho 衍生化合物可能具有作为口腔卫生产品添加剂的潜力。

更新日期：2020-06-18

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