The restricted bioavailability of structurally complex carbohydrates for digestion has hitherto resulted in a low methane potential from rubber wood waste (RW). The effects of hydrothermal (HT) and ethanol organosolv (OS) pretreatments on the methane produced by anaerobic digestion of RW were investigated in the study reported. HT with temperatures above 190 °C significantly enhanced the anaerobic digestibility of RW mainly due to the degradation of hemicellulose. On the other hand, OS with 75% ethanol provided a potential methane gas yield of 165.1 L CH₄/kg-VS, which was higher than that for HT at 210 and 230 °C by 39 and 7%, respectively. This was due to intensive delignification during OS pretreatment which led to a reduction in the non-productive adsorption of cellulolytic enzymes by lignin. A first-order kinetic model showed that OS had a higher hydrolysis rate (k = 0.073 ± 0.003 day⁻¹) resulting in a higher methane yield when lower pretreatment temperatures were applied. The anaerobic degradation of the pretreated RW in this study was a result of simultaneous CH₄ production through the symbiosis of anaerobic bacteria and methanogens using a combination of the aceticlastic and hydrogenothrophic bioconversion pathways. The recovery and use of the phenolic compounds remaining in the process water would be a way of adding value to this process and the feasibility of producing methane from RW should be further investigated.

迄今为止，结构复杂的碳水化合物用于消化的生物利用度受到限制，导致橡胶木废料（RW）产生的甲烷潜力较低。在研究报告中研究了水热（HT）和乙醇有机溶剂（OS）预处理对RW厌氧消化产生的甲烷的影响。温度高于190°C的HT显着提高了RW的厌氧消化率，这主要是由于半纤维素的降解。另一方面，含75％乙醇的OS可提供165.1 L CH ₄的潜在甲烷气产量/ kg-VS，分别比210和230°C的HT高39和7％。这是由于在OS预处理过程中强烈的去木质素作用，导致木质素对纤维素分解酶的非生产性吸附减少。一级动力学模型显示OS具有更高的水解速率（k  = 0.073±0.003天^-1），当采用较低的预处理温度时，其甲烷产率更高。这项研究中预处理的RW的厌氧降解是同时发生CH ₄的结果通过结合使用抗微生物和氢致营养生物转化途径，通过厌氧细菌和产甲烷菌的共生来生产微生物。回收和使用工艺用水中残留的酚类化合物将是该工艺增值的一种方式，应进一步研究从RW制甲烷的可行性。