The use of plant growth-promoting rhizobacteria (PGPR) is increasingly meaningful for the development of more environmentally friendly agricultural practices. However, often the PGPR strains selected in the laboratory fail to confer the expected beneficial effects when evaluated in plant experiments. Insufficient rhizosphere colonization is pointed out as one of the causes. With the aim of minimizing this inconsistency, we propose that besides studying plant growth promotion traits (PGP), the screening strategy should include evaluation of the microbial phenotypes required for colonization and persistence. As a model, we carried out this strategy in three Rhizobium sp. strains that showed phosphorus solubilization ability and production of siderophores. All strains displayed colonization phenotypes like surface spreading, resistance to hydrogen peroxide, and formed biofilms. Regarding their ability to persist, biofilm formation was observed to be influenced by pH and the phosphorus nutrient provided in the growth media. Differences in the competence of the strains to use several carbon substrates were also detected. As part of our framework, we compared the phenotypic characteristics of the strains in a quantitative manner. The data analysis was integrated using a multicriteria decision analysis (MCDA). All our results were scored, weighted, and grouped as relevant for PGP, colonization, or persistence. MCDA demonstrated that, when the phenotypes related to PGP and colonization are weighted over those for persistence, strain B02 performs better than the other two Rhizobium sp. strains. The use of our framework could assist the selection of more competent strains to be tested in greenhouse and field trials.

中文翻译：

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基于细菌竞争机制选择促进植物生长的根际细菌的框架。

促进植物生长的根际细菌（PGPR）的使用对于发展更环保的农业实践越来越有意义。但是，在植物实验中评估时，实验室中选择的PGPR菌株通常无法赋予预期的有益效果。指出根际定植不足是原因之一。为了最大程度地减少这种不一致，我们建议除了研究植物生长促进性状（PGP）外，筛选策略还应包括评估定植和持久性所需的微生物表型。作为模型，我们在三个根瘤菌中实施了该策略sp。菌株显示出磷的溶解能力和铁载体的产生。所有菌株均表现出定殖表型，如表面扩散，对过氧化氢的抗性和形成的生物膜。关于它们的持久能力，观察到生物膜的形成受pH和生长培养基中提供的磷营养物的影响。还检测了菌株使用几种碳底物的能力差异。作为我们框架的一部分，我们以定量方式比较了菌株的表型特征。使用多准则决策分析（MCDA）集成了数据分析。我们对所有结果进行评分，加权和分组，以与PGP，定植或持久性相关。MCDA证明，根瘤菌属。株。使用我们的框架可以帮助选择更有能力的菌株进行温室和田间试验。