Artificial sweeteners have been shown to induce glucose intolerance by altering the gut microbiota; however, little is known about the effect of stevia. Here, we investigate whether stevia supplementation induces glucose intolerance by altering the gut microbiota in mice, hypothesizing that stevia would correct high fat diet-induced glucose intolerance and alter the gut microbiota. Mice were split into four treatment groups: low fat, high fat, high fat + saccharin and high fat + stevia. After 10 weeks of treatment, mice consuming a high fat diet (60% kcal from fat) developed glucose intolerance and gained more weight than mice consuming a low fat diet. Stevia supplementation did not impact body weight or glucose intolerance. Differences in species richness and relative abundances of several phyla were observed in low fat groups compared to high fat, stevia and saccharin. We identified two operational taxonomic groups that contributed to differences in beta-diversity between the stevia and saccharin groups: Lactococcus and Akkermansia in females and Lactococcus in males. Our results demonstrate that stevia does not rescue high fat diet-induced changes in glucose tolerance or the microbiota, and that stevia results in similar alterations to the gut microbiota as saccharin when administered in concordance with a high fat diet.

中文翻译：

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在饮食诱导的肥胖小鼠模型中，甜菊对肠道菌群和葡萄糖耐量的影响。

人造甜味剂已显示出通过改变肠道菌群而引起葡萄糖耐受不良。然而，关于甜叶菊的作用知之甚少。在这里，我们研究甜叶菊补充剂是否通过改变小鼠的肠道菌群来诱导葡萄糖耐量降低，假设甜菊糖可以纠正高脂饮食诱导的葡萄糖不耐症并改变肠道菌群。将小鼠分为四个治疗组：低脂，高脂，高脂+糖精和高脂+甜叶菊。治疗10周后，与高脂饮食的小鼠相比，高脂饮食（60％的卡路里来自脂肪）的小鼠出现葡萄糖耐受不良，体重增加。甜叶菊补充剂不会影响体重或葡萄糖耐量。与高脂，甜叶菊和糖精相比，低脂人群中几种门的物种丰富度和相对丰度有所不同。我们确定了两个导致甜叶菊糖精和糖精蛋白之间的β-多样性差异的可操作分类学组：女性乳球菌和阿克曼酵母菌，男性乳球菌。我们的结果表明，甜菊糖不能挽救高脂饮食引起的葡萄糖耐量或微生物群的变化，并且与高脂饮食配合服用时，甜菊糖会导致糖精与肠道菌群发生相似的改变。