Alopecia areata is an autoimmune disease in which activation of autoreactive T cells and inflammatory immune signals target the hair follicles autoantigens. Although cytokines are involved in regulating autoimmune inflammation, the specific involvement of these molecules in the pathogenesis of alopecia areata has been remained unsettled. Here, a possible influence of IL12B, IL17A, and IL23R variations on susceptibility to alopecia areata in Iranian patients was investigated. Genotyping of IL12B (rs3212227), IL17A (rs2275913), and IL23R (rs10889677) variants were performed by extracting genomic DNA from patients and controls. Gene expression was analyzed by real-time RT-PCR. The frequency of IL12B and IL23R gene polymorphisms is significantly higher in the patients than controls, while no significant difference was found for IL17A. Stratification of the patients with respect to age at disease onset indicated that CC genotype of IL12B (rs3212227) and AA genotype of IL23R (rs10889677) gene polymorphisms are significantly associated with late-onset alopecia areata disease. In contrast to IL17A and IL23R, IL12B gene expression levels elevated in patients to that of controls, but genotypes had no effect on levels of gene expression. Overall, our data confirmed that the IL12B and IL23R polymorphisms are associated with the risk to develop alopecia areata in our population.

中文翻译：

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IL12B和IL23R多态性与斑秃有关。

斑秃是一种自身免疫性疾病，其中自身反应性T细胞的激活和炎性免疫信号靶向毛囊自身抗原。尽管细胞因子参与调节自身免疫炎症，但是这些分子在斑秃的发病机理中的具体参与仍未确定。在此，研究了IL12B，IL17A和IL23R变化对伊朗患者对斑秃的敏感性的可能影响。IL12B（rs3212227），IL17A（rs2275913）和IL23R（rs10889677）变体的基因分型是通过从患者和对照中提取基因组DNA来进行的。通过实时RT-PCR分析基因表达。患者中IL12B和IL23R基因多态性的频率显着高于对照组，而IL17A没有发现显着差异。根据发病年龄对患者进行分层表明，IL12B的CC基因型（rs3212227）和IL23R的AA基因型（rs10889677）基因多态性与迟发性斑秃有显着相关性。与IL17A和IL23R相反，患者中IL12B基因表达水平高于对照组，但基因型对基因表达水平没有影响。总体而言，我们的数据证实IL12B和IL23R多态性与我们人群中发生斑秃的风险有关。患者中IL12B基因表达水平高于对照组，但基因型对基因表达水平没有影响。总体而言，我们的数据证实IL12B和IL23R多态性与我们人群中发生斑秃的风险有关。患者中IL12B基因表达水平高于对照组，但基因型对基因表达水平没有影响。总体而言，我们的数据证实IL12B和IL23R多态性与我们人群中发生斑秃的风险有关。