Intense synthesis of proteins and chemicals in engineered microbes impose metabolic burden, frequently leading to reduced growth and heterogeneous cell population. Thus, the correct balance between growth and production is important. Such balance can be engineered through dynamic control of pathways, but few broadly applicable tools are available to achieve this. We present an autonomous control of gene expression mediated by quorum sensing in Bacillus subtilis, able to self-monitor and induce expression without human supervision. Two variations of the induction module and seven of the response module were engineered generating a range of induction folds and strengths for gene expression control. Our strongest response promoter is 2.5 and 3.2 times stronger than the well-characterized promoters P_srfA and P_veg, respectively. We applied our strongest autoinduction device for the production of the vitamin B₂. This study presents a toolbox of autoinduction modules for B. subtilis that is modular and tunable.

工程微生物中蛋白质和化学物质的密集合成会带来代谢负担，经常导致生长减少和细胞数量异质化。因此，增长和生产之间的正确平衡很重要。这种平衡可以通过路径的动态控制来设计，但很少有广泛适用的工具可以实现这一点。我们在枯草芽孢杆菌中提出了由群体感应介导的基因表达的自主控制，能够在没有人类监督的情况下自我监测和诱导表达。诱导模块的两个变体和响应模块的七个变体被设计为产生一系列用于基因表达控制的诱导折叠和强度。我们最强的响应启动子比充分表征的启动子 P 强 2.5 和 3.2 倍_srfA和 P _veg，分别。我们应用了我们最强的自动感应装置来生产维生素 B ₂。本研究为枯草芽孢杆菌提供了一个模块化和可调的自动感应模块工具箱。